doi:info:doi/10.1016/j.bmcl.2017.07.057

Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye 利用統計を見る

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BMCL27_4024.pdf
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アイテムタイプ	学術雑誌論文 = Journal Article
言語	英語
キーワード	Far-red squaraine dye, Aggregation, Fluorescent peptide, Self-quenching, Dye-peptide conjugate, Elastase detection, Bio imaging
著者	Saikiran Maryala Sato Daisuke Pandey Shyam S. Hayase Shuzi Kato Tamaki
著者所属	Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology
抄録	Extended wavelength analyte-responsive fluorescent probes are highly desired for the imaging applications owing to their deep tissue penetration, and minimum interference from autofluorescence by biomolecules. Near infra-red (NIR) sensitive and self-quenching fluorescent probe based on the dye-peptide conjugate (SQ 1 PC) was designed and synthesized by facile and efficient one-pot synthetic route for the detection of Elastase activity. In the phosphate buffer solution, there was an efficient quenching of fluorescence of SQ 1 PC (86%) assisted by pronounced dye-dye interaction due to H-aggregate formation. Efficient and fast recovery of this quenched fluorescence of SQ 1 PC (> 50% in 30 s) was observed on hydrolysis of this peptide-dye conjugate by elastase enzyme. Presently designed NIR sensitive self-quenching substrate offers the potential application for the detection of diseases related to proteases by efficient and fast detection of their activities.
雑誌名	Bioorganic & Medicinal Chemistry Letters
巻	27
号	17
ページ	4024 - 4029
発行年	2017-07-22
出版者	Elsevier
ISSN	0960-894X 1464-3405
書誌レコードID	AA1079577X
DOI	info:doi/10.1016/j.bmcl.2017.07.057
権利	Copyright (c) 2017 Elsevier Ltd.
情報源	https://doi.org/10.1016/j.bmcl.2017.07.057
版	author
査読の有無	yes
資料タイプ	Journal Article
研究者情報	https://hyokadb02.jimu.kyutech.ac.jp/html/332_ja.html
連携ID	6623

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Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye 利用統計を見る

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Kyushu Institute of Technology Academic Repository

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Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye 利用統計を見る

Kyutacar　九州工業大学学術機関リポジトリ