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新規フェロセン化ナフタレンジイミドを用いる均一溶液中でのシグナルオン型電気化学的DNase I検出
http://hdl.handle.net/10228/00007618
http://hdl.handle.net/10228/0000761895994934-ec67-4df7-89ef-5eb4ebe668a1
名前 / ファイル | ライセンス | アクション |
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bunsekikagaku.68.953.pdf (1.1 MB)
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Item type | 学術雑誌論文 = Journal Article(1) | |||||||||||||||||
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公開日 | 2020-02-17 | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | journal article | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | ja | |||||||||||||||||
タイトル | 新規フェロセン化ナフタレンジイミドを用いる均一溶液中でのシグナルオン型電気化学的DNase I検出 | |||||||||||||||||
その他のタイトル | ||||||||||||||||||
その他のタイトル | Signal-on Type Electrochemical Detection of DNase I in Homogeneous Media Using New Ferrocenylnaphthalene Diimide | |||||||||||||||||
言語 | en | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | jpn | |||||||||||||||||
著者 |
佐藤, しのぶ
× 佐藤, しのぶ
WEKO
5963
× 福瀧, 修司× 竹中, 繁織
WEKO
6749
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抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | A simple DNase I detecting method was achieved under a homogenous medium containing DNA duplex and a newly synthesized ferrocenylnaphthalene diimide derivative (FND) carrying alanine (1) or alanine-lysine residue (2) as an electrochemical indicator. The binding affinity of 1 or 2 with double stranded DNA was 105 M−1 or 106 M−1 order, which was estimated from a Scatchard analysis calculated by its absorption change upon the addition of calf thymus DNA (CT-DNA). It was expected that 1 or 2 intercalated to double stranded DNA with a threading mode and electrostatic interaction of the linker ammonium cations of 1 or 2 with phosphate anions of the DNA duplex backbone. The largest current increase after a DNase I treatment was observed under the mixture in a ratio of [3]:[CT-DNA-bp] = 10 : 1 (bp: DNA concentration per base pair), where the distance between the bound ligands is expected to a 10 bp or 34 Å theoretically. When considering to be ca. 30 Å of DNase I size and decreasing of the current increase under an increased amount of 3, DNase I required over ten base pairs to access and digest with the DNA duplex. The current increase in the case of a mixture of 10 μM 1 or 2 and 100 μM CT-DNA was obtained 340 % or 640 %, respectively, after being treated with DNase I (final concentration: 2.0 × 10−3 U μL−1), which might be derived from an apperarent molecular weight or diffusion coefficient of the ligand bound DNA duplex and the detection limit of DNase I in the case of 1 or 2 was 2.0 × 10−5 or 2.0 × 10−9 U μL−1, respectively, where the detection limit for 2 was 104-times higher than that for 1. | |||||||||||||||||
書誌情報 |
分析化学 巻 68, 号 12, p. 953-960, 発行日 2019-12-05 |
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出版社 | ||||||||||||||||||
出版者 | 日本分析化学会 | |||||||||||||||||
DOI | ||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||
関連識別子 | info:doi/10.2116/bunsekikagaku.68.953 | |||||||||||||||||
論文ID(NAID) | ||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||
識別子タイプ | NAID | |||||||||||||||||
関連識別子 | 130007783471 | |||||||||||||||||
NCID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AN00222633 | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 0525-1931 | |||||||||||||||||
著作権関連情報 | ||||||||||||||||||
権利情報 | Copyright (c) 2019 The Japan Society for Analytical Chemistry | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | amino acid residues | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | DNase I | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | electrochemical detection | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | ferrocenylnaphthalene diimide | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | homogenous medium | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | signal-on type detection | |||||||||||||||||
出版タイプ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
査読の有無 | ||||||||||||||||||
値 | yes | |||||||||||||||||
連携ID | ||||||||||||||||||
8125 | ||||||||||||||||||
資料タイプ | ||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||
内容記述 | Journal Article | |||||||||||||||||
著者別名 | ||||||||||||||||||
姓名 | Sato, Shinobu | |||||||||||||||||
言語 | en | |||||||||||||||||
姓名 | 佐藤, しのぶ | |||||||||||||||||
言語 | ja | |||||||||||||||||
姓名 | サトウ, シノブ | |||||||||||||||||
言語 | ja-Kana | |||||||||||||||||
著者別名 | ||||||||||||||||||
姓名 | Fukutaki, Shuji | |||||||||||||||||
言語 | en | |||||||||||||||||
著者別名 | ||||||||||||||||||
姓名 | Takenaka, Shigeori | |||||||||||||||||
言語 | en | |||||||||||||||||
姓名 | 竹中, 繁織 | |||||||||||||||||
言語 | ja | |||||||||||||||||
姓名 | タケナカ, シゲオリ | |||||||||||||||||
言語 | ja-Kana | |||||||||||||||||
著者所属 | ||||||||||||||||||
九州工業大学大学院工学研究院物質工学研究系 804-8550福岡県北九州市戸畑区仙水町1-1, 九州工業大学バイオマイクロセンシング技術研究センター | ||||||||||||||||||
著者所属 | ||||||||||||||||||
九州工業大学大学院工学研究院物質工学研究系 804-8550福岡県北九州市戸畑区仙水町1-1 | ||||||||||||||||||
著者所属 | ||||||||||||||||||
九州工業大学大学院工学研究院物質工学研究系 804-8550福岡県北九州市戸畑区仙水町1-1, 九州工業大学バイオマイクロセンシング技術研究センター | ||||||||||||||||||
情報源 | ||||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||
関連識別子 | https://doi.org/10.2116/bunsekikagaku.68.953 | |||||||||||||||||
関連名称 | https://doi.org/10.2116/bunsekikagaku.68.953 |