{"created":"2024-04-12T05:40:28.277174+00:00","id":2000502,"links":{},"metadata":{"_buckets":{"deposit":"95b98efa-cb3a-4bc3-a04d-5606cddb469b"},"_deposit":{"created_by":14,"id":"2000502","owner":"14","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"2000502"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:02000502","sets":["8:9"]},"author_link":["3998"],"control_number":"2000502","item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-16","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"Cell and Tissue Research","bibliographic_titleLang":"en"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sour taste is detected by type III taste receptor cells that generate membrane depolarization with action potentials in response to HCl applied to the apical membranes. The shape of action potentials in type III cells exhibits larger afterhyperpolarization due to activation of transient A-type voltage-gated K+ currents. Although action potentials play an important role in neurotransmitter release, the electrophysiological features of A-type K+ currents in taste buds remain unclear. Here, we examined the electrophysiological properties of A-type K+ currents in mouse fungiform taste bud cells using in-situ whole-cell patch clamping. Type III cells were identified with SNAP-25 immunoreactivity and/or electrophysiological features of voltage-gated currents. Type III cells expressed A-type K+ currents which were completely inhibited by 10 mM TEA, whereas IP3R3-immunoreactive type II cells did not. The half-maximal activation and steady-state inactivation of A-type K+ currents were 17.9 ± 4.5 (n = 17) and − 11.0 ± 5.7 (n = 17) mV, respectively, which are similar to the features of Kv3.3 and Kv3.4 channels (transient and high voltage-activated K+ channels). The recovery from inactivation was well fitted with a double exponential equation; the fast and slow time constants were 6.4 ± 0.6 ms and 0.76 ± 0.26 s (n = 6), respectively. RT-PCR experiments suggest that Kv3.3 and Kv3.4 mRNAs were detected at the taste bud level, but not at single-cell levels. As the phosphorylation of Kv3.3 and Kv3.4 channels generally leads to the modulation of cell excitability, neuromodulator-mediated A-type K+ channel phosphorylation likely affects the signal transduction of taste.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_21_link_62":{"attribute_name":"研究者情報","attribute_value_mlt":[{"subitem_link_url":"https://hyokadb02.jimu.kyutech.ac.jp/html/361_ja.html"}]},"item_21_publisher_7":{"attribute_name":"出版社","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00441-024-03887-6","subitem_relation_type_select":"DOI"}}]},"item_21_rights_13":{"attribute_name":"著作権関連情報","attribute_value_mlt":[{"subitem_rights":"Copyright (c) The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.","subitem_rights_resource":"http://creativecommons.org/licenses/by/4.0/"}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_10":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00600105","subitem_source_identifier_type":"NCID"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0302-766X","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"1432-0878","subitem_source_identifier_type":"EISSN"}]},"item_21_text_28":{"attribute_name":"論文ID（連携）","attribute_value_mlt":[{"subitem_text_value":"10429742"}]},"item_21_text_63":{"attribute_name":"連携ID","attribute_value_mlt":[{"subitem_text_value":"12089"}]},"item_21_version_type_58":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Moribayashi, Takeru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakao, Yoshiki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Otsubo, Yoshitaka","creatorNameLang":"en"},{"creatorName":"大坪, 義孝","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-04-12"}],"filename":"10429742.pdf","filesize":[{"value":"4.8 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kyutech.repo.nii.ac.jp/record/2000502/files/10429742.pdf"},"version_id":"06540f5c-d57d-4094-b154-e58aad62b486"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"In-situ whole-cell patch clamping","subitem_subject_scheme":"Other"},{"subitem_subject":"Voltage-gated potassium currents","subitem_subject_scheme":"Other"},{"subitem_subject":"Fungiform taste buds","subitem_subject_scheme":"Other"},{"subitem_subject":"Sour-sensing cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Excitability","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characteristics of A-type voltage-gated K+ currents expressed on sour-sensing type III taste receptor cells in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characteristics of A-type voltage-gated K+ currents expressed on sour-sensing type III taste receptor cells in mice","subitem_title_language":"en"}]},"item_type_id":"21","owner":"14","path":["9"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-12"},"publish_date":"2024-04-12","publish_status":"0","recid":"2000502","relation_version_is_last":true,"title":["Characteristics of A-type voltage-gated K+ currents expressed on sour-sensing type III taste receptor cells in mice"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-04-12T05:51:21.038342+00:00"}