WEKO3
アイテム
Substrates incorporating unnatural amino acids are separately detectable for chymotrypsin and subtilisin with similar specificity
http://hdl.handle.net/10228/0002001131
http://hdl.handle.net/10228/000200113186c371ff-d0a4-4d4b-aac8-56bf711ef7f6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10443877.pdf (4.4 MB)
|
|
| アイテムタイプ | 共通アイテムタイプ(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-01-20 | |||||||
| タイトル | ||||||||
| タイトル | Substrates incorporating unnatural amino acids are separately detectable for chymotrypsin and subtilisin with similar specificity | |||||||
| 言語 | en | |||||||
| 著者 |
Yamawaki, Yuuki
× Yamawaki, Yuuki
× 加藤, 珠樹 |
|||||||
| 著作権関連情報 | ||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by-n nc-nd/4.0/ | |||||||
| 権利情報 | Copyright(c) 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-n nc-nd/4.0/ | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Protease specificity depends on the three-dimensional structure and sequence of the protease, and each protease plays a different role. Chymotrypsin and subtilisin have similar specificity for recognizing aromatic and hydrophobic amino acid residues. Previous methods using natural amino acids have difficulty in separately detecting proteases with similar specificity. To overcome this, we developed an effective substrate incorporating unnatural amino acids using a simple method that changes only one residue of a substrate. Various 7-amino-4-methylcoumarin (AMC) peptide substrates were synthesized in terms of hydrophobicity, aromaticity, and conformation, including unnatural amino acids 2-amino-4-ethylhexanoic acid (AEH) and cyclohexylalanine (Cha). Although AEH substrate is highly hydrophobic, steric hindrance based on the branched side chain of AEH residue reduced the recognition of the substrate by the two proteases. Cha substrate with a six-membered ring side chain was most preferably recognized by chymotrypsin but was slightly recognized by subtilisin. This shows that Cha substrate can separately detect the two proteases with similar specificity. Therefore, these results indicate that protease with similar specificities is separately detectable by substrates with a side chain having structural degrees of freedom that provide different steric hindrance. | |||||||
| 言語 | en | |||||||
| 書誌情報 |
en : Results in Chemistry 巻 6, p. 101217, 発行日 2023-11-23 |
|||||||
| 出版社 | ||||||||
| 出版者 | Elsevier | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1016/j.rechem.2023.101217 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 2211-7156 | |||||||
| 査読の有無 | ||||||||
| 値 | yes | |||||||
| 研究者情報 | ||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/326_ja.html | |||||||
| 論文ID（連携） | ||||||||
| 値 | 10443877 | |||||||
| 連携ID | ||||||||
| 値 | 12675 | |||||||
