| Item type |
共通アイテムタイプ(1) |
| 公開日 |
2025-01-28 |
| タイトル |
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|
タイトル |
Biotinylated cyclic naphthalene diimide as a searching tool for G4 sites on the genome |
|
言語 |
en |
| 著者 |
藤井, 聡
佐藤, しのぶ
Hidaka, Ryuki
竹中, 繁織
|
| 著作権関連情報 |
|
|
権利情報 |
Copyright (c) The Author(s) 2024 |
| 著作権関連情報 |
|
|
権利情報Resource |
http://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A biotinyl cyclic naphthalene diimide (biotinyl cNDI) (1), in which biotin is introduced on the cyclic linker chain of cNDI with high G-quadruplex (G4) specificity, was synthesized. 1 was used for binding analysis to G4 DNAs such as c-myc, c-kit, CEGF, or TA-core. The results showed that 1 bind to G4 DNAs with high affinity and, especially, two molecules of 1 bind to c-myc DNA from top and bottom of G4 site at K = 3.9 × 10−6 M−1 without changing the G4 structure. As a pulldown assay, 1 and streptavidin magnetic beads could be used to recover a c-myc DNA or 120-mer DNA fragment having single c-myc sequence. The qPCR results for the 120-meric DNAs showed that more than 50% of genomic DNA fragments could be recovered by this pulldown assay. The results obtained here might allow the recovery of G4-containing DNA fragments from genomic DNA to analyze the true G4 present in the genome. |
|
言語 |
en |
| 書誌情報 |
en : Analytical Sciences
巻 40,
号 5,
p. 943-950,
発行日 2024-04-12
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| 出版社 |
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出版者 |
Springer |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
G-quadruplex |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Cyclic naphthalene diimide (cNDI) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Isothermal titration calorimetry (ITC) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Pulldown assay |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Quantitative polymerase chain reaction (qPCR) |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s44211-024-00551-5 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10500785 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0910-6340 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1348-2246 |
| 査読の有無 |
|
|
値 |
yes |
| 研究者情報 |
|
|
URL |
https://hyokadb02.jimu.kyutech.ac.jp/html/287_ja.html |
| 研究者情報 |
|
|
URL |
https://hyokadb02.jimu.kyutech.ac.jp/html/395_ja.html |
| 論文ID（連携） |
|
|
値 |
10443357 |
| 連携ID |
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|
値 |
12503 |
10443357.pdf (939 KB)
