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アイテム
Two-layered Microwell-array Device for Preparation of Single-neuron Culture Samples
http://hdl.handle.net/10228/0002001200
http://hdl.handle.net/10228/0002001200d0754a55-dd0e-4659-b8df-557b15c0b520
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10444304.pdf (1 MB)
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| アイテムタイプ | 共通アイテムタイプ(1) | |||||||||||
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| 公開日 | 2025-01-31 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Two-layered Microwell-array Device for Preparation of Single-neuron Culture Samples | |||||||||||
| 言語 | en | |||||||||||
| その他のタイトル | ||||||||||||
| その他のタイトル | Two-layered microwell-array device for preparation of single-neuron culture samples | |||||||||||
| 言語 | en | |||||||||||
| 著者 |
Nakama, Ayaka
× Nakama, Ayaka
× 安田, 隆
WEKO
24966
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| 著作権関連情報 | ||||||||||||
| 権利情報 | Copyright (c) 2024 IOP Publishing Ltd | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | When a single neuron is cultured in isolation from other neurons, its axon connects with its own dendrites to form a simple, independent network with no synaptic inputs from other neurons. This culture system enables detailed analysis of synaptic function and morphology change in neurites at the single-neuron level, which is useful for elucidating the pathogenesis of neurological diseases and for evaluating the efficacy of therapeutic drugs for them. However, there was previously no device technology capable of simultaneously forming multiple single-neuron samples while allowing co-culture with astrocytes, which is essential for culture of a single neuron isolated from other neurons. In this study, we propose a novel microwell-array device for preparing single-neuron samples. The device consists of an upper layer for cell seeding and a lower layer for cell culture. Each layer has 16 × 16 microwells, and the bottom of each well is made of a 1 μm thick silicon nitride membrane. The membrane of the upper well has one microhole for seeding a single neuron, and the lower membrane has multiple microholes for interaction between a single neuron and astrocytes which are co-cultured back-to-back on both sides of the membrane. When neurons are seeded into the upper well, only one of them passes through the microhole in the upper membrane and falls onto the lower membrane. We evaluated a seeding efficiency of single neurons by changing seeding hole diameter and seeding density. The results showed that the yield of more than 20% was obtained regardless of the seeding density when the seeding hole diameter was 13 μm. We also confirmed that single neurons seeded in this manner and co-cultured with astrocytes developed neurites and formed synapses. These results demonstrated the usefulness of this device for the preparation of single-neuron culture samples. | |||||||||||
| 言語 | en | |||||||||||
| 書誌情報 |
en : Journal of Micromechanics and Microengineering 巻 34, 号 8, p. 085011, 発行日 2024-06-24 |
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| 出版社 | ||||||||||||
| 出版者 | IOP Publishing | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | single neuron | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | silicon nitride | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | microwell | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | microhole | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | co-culture | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1088/1361-6439/ad5b00 | |||||||||||
| NCID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA10804010 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 0960-1317 | |||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||
| 収録物識別子 | 1361-6439 | |||||||||||
| 査読の有無 | ||||||||||||
| 値 | yes | |||||||||||
| 研究者情報 | ||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/325_ja.html | |||||||||||
| 論文ID（連携） | ||||||||||||
| 値 | 10444304 | |||||||||||
| 連携ID | ||||||||||||
| 値 | 12581 | |||||||||||
