| アイテムタイプ |
共通アイテムタイプ(1) |
| 公開日 |
2025-02-03 |
| タイトル |
|
|
タイトル |
Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme |
|
言語 |
en |
| 著者 |
Gupta, Shekhar
Balyan, Priyanka
Mavileti, Sai Kiran
パンディ, シャム スディル
加藤, 珠樹
|
| 著作権関連情報 |
|
|
権利情報Resource |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
Copyright(c) 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Chymotrypsin, a crucial enzyme in human digestion, catalyzes the breakdown of milk proteins, underscoring its significance in both health diagnostics and dairy quality assurance. Addressing the critical need for rapid, cost-effective detection methods, we introduce a groundbreaking approach utilizing far-red technology and HOMO-Förster resonance energy transfer (FRET). Our novel probe, SQ-122 PC, features a unique molecular design that includes a squaraine dye (SQ), a peptide linker, and SQ moieties synthesized through solid-phase peptide synthesis. Demonstrating a remarkable quenching efficiency of 93.75% in a tailored H2O:DMSO (7:3) solvent system, our probe exhibits absorption and emission properties within the far-red spectrum, with an unprecedented detection limit of 0.130 nM. Importantly, our method offers unparalleled selectivity towards chymotrypsin, ensuring robust and accurate enzyme detection. This pioneering work underscores the immense potential of far-red-based homo-FRET systems in enabling the sensitive and specific detection of chymotrypsin enzyme activity. By bridging the gap between cutting-edge technology and biomedical diagnostics, our findings herald a new era of enzyme sensing, promising transformative advancements in disease diagnosis and dairy quality control. |
|
言語 |
en |
| 書誌情報 |
en : Molecules
巻 29,
号 6,
p. 1282,
発行日 2024-04-14
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| 出版社 |
|
|
出版者 |
MDPI |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
homo-FRET |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
dye–peptide conjugate |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
chymotrypsin detection |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
far-red squaraine dye |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
fluorescent peptides |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
fluorescence microscopy |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/molecules29061282 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1420-3049 |
| 査読の有無 |
|
|
値 |
yes |
| 研究者情報 |
|
|
URL |
https://hyokadb02.jimu.kyutech.ac.jp/html/326_ja.html |
| 論文ID（連携） |
|
|
値 |
10443875 |
| 連携ID |
|
|
値 |
12673 |
10443875.pdf (1.9 MB)
