WEKO3
アイテム
Cell surface display of a protein based on a tail-anchored membrane protein
http://hdl.handle.net/10228/0002001589
http://hdl.handle.net/10228/0002001589cad3d92c-b924-4ca0-8fbd-fa3c7a8bc5d5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10450362.pdf (2.5 MB)
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| アイテムタイプ | 共通アイテムタイプ(1) | |||||||||||||||||
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| 公開日 | 2025-04-15 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Cell surface display of a protein based on a tail-anchored membrane protein | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 著者 |
末田, 慎二
× 末田, 慎二
WEKO
958
× Tsuruga, Rima
× Hirakawa, Takumi
× 藤井, 聡
WEKO
15863
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| 著作権関連情報 | ||||||||||||||||||
| 権利情報 | Copyright (c) 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Methods for displaying proteins on the cell surface are widely used in protein-based biotechnology and bioengineering, where target proteins are expressed as fusion constructs with membrane proteins through recombinant DNA technology. In this study, we developed a system for displaying a protein on the cell surface using the transmembrane domain (TMD) of a tail-anchored membrane protein (TA protein). TA proteins have an orientation in the cell membrane such that their C-termini are displayed on the cell surface, which contrasts with that of type I transmembrane proteins that are commonly used as anchoring units. Therefore, by utilizing the TMD of a TA protein as an anchoring unit, desired proteins can be attached to the TMD via their N-termini. This approach is advantageous for displaying proteins whose C-terminal regions play important roles in their activity. In this study, we chose the inner nuclear membrane protein emerin as a TA protein and constructed expression systems in mammalian cells for a series of fusion proteins based on deleted forms of emerin. We found that utilizing emerin that lacks 210 residues from the N-terminus as a TMD allowed efficient translocation of the fusion protein to the plasma membrane, successfully displaying its target protein portion on the cell surface. Thus, our system serves as an effective method for protein display, enhancing the applicability of cell surface display technology based on transmembrane proteins. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : Biochemical and Biophysical Research Communications 巻 761, p. 151738, 発行日 2025-04-05 |
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| 出版社 | ||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Protein cell surface display | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Tail-anchored membrane protein | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Emerin | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Transmembrane protein | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
| DOI | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.bbrc.2025.151738 | |||||||||||||||||
| 助成情報 | ||||||||||||||||||
| 助成機関識別子タイプ | Crossref Funder | |||||||||||||||||
| 助成機関識別子 | https://doi.org/10.13039/501100001691 | |||||||||||||||||
| 助成機関名 | 日本学術振興会 (JSPS) | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| 助成機関名 | Japan Society for the Promotion of Science (JSPS) | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 研究課題番号タイプ | JGN | |||||||||||||||||
| 研究課題番号 | 24K08481 | |||||||||||||||||
| 研究課題番号URI | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K08481/ | |||||||||||||||||
| 研究課題名 | 特異な酵素反応を利用した細胞表層でのATP検出技術の開発 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||
| 収録物識別子 | 0006-291X | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||
| 収録物識別子 | 1090-2104 | |||||||||||||||||
| 査読の有無 | ||||||||||||||||||
| 値 | yes | |||||||||||||||||
| 研究者情報 | ||||||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/280_ja.html | |||||||||||||||||
| 研究者情報 | ||||||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/287_ja.html | |||||||||||||||||
| 論文ID（連携） | ||||||||||||||||||
| 値 | 10450362 | |||||||||||||||||
| 連携ID | ||||||||||||||||||
| 値 | 14314 | |||||||||||||||||
