| アイテムタイプ |
共通アイテムタイプ(1) |
| 公開日 |
2025-08-01 |
| タイトル |
|
|
タイトル |
NIR-Sensitive Squaraine Dye—Peptide Conjugate for Trypsin Fluorogenic Detection |
|
言語 |
en |
| 著者 |
Priyanka Balyan,
Shekhar Gupta,
Sai Kiran Mavileti,
パンディ, シャム スディル
加藤, 珠樹
|
| 著作権関連情報 |
|
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
|
言語 |
en |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Trypsin enzyme has gained recognition as a potential biomarker in several tumors, such as colorectal, gastric, and pancreatic cancer, highlighting its importance in disease diagnosis. In response to the demand for rapid, cost-effective, and real-time detection methods, we present an innovative strategy utilizing the design and synthesis of NIR-sensitive dye–peptide conjugate (SQ-3 PC) for the sensitive and selective monitoring of trypsin activity by fluorescence ON/OFF sensing. The current research deals with the design and synthesis of three unsymmetrical squaraine dyes SQ-1, SQ-2, and SQ-3 along with a dye–peptide conjugate SQ-3-PC as a trypsin-specific probe followed by their photophysical characterizations. The absorption spectral investigation conducted on both the dye alone and its corresponding dye–peptide conjugates in water, utilizing SQ-3 and SQ-3 PC respectively, reveals enhanced dye aggregation and pronounced fluorescence quenching compared to observations in DMSO solution. The absorption spectral investigation conducted on dye only and corresponding dye–peptide conjugates in water utilizing SQ-3 and SQ-3 PC, respectively, reveals not only the enhanced dye aggregation but also pronounced fluorescence quenching compared to that observed in the DMSO solution. The trypsin-specific probe SQ-3 PC demonstrated a fluorescence quenching efficiency of 61.8% in water attributed to the combined effect of aggregation-induced quenching (AIQ) and fluorescence resonance energy transfer (FRET). FRET was found to be dominant over AIQ. The trypsin-mediated hydrolysis of SQ-3 PC led to a rapid and efficient recovery of quenched fluorescence (5-fold increase in 30 min). Concentration-dependent changes in the fluorescence at the emission maximum of the dyes reveal that SQ-3 PC works as a trypsin enzyme-specific fluorescence biosensor with linearity up to 30 nM along with the limit of detection and limit of quantification of 1.07 nM and 3.25 nM, respectively. |
|
言語 |
en |
| 書誌情報 |
en : Biosensors
巻 14,
号 10,
p. 458,
発行日 2024-09-25
|
| 出版社 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
trypsin detection |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
aggregation-caused quenching |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
FRET |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NIR detection |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
squaraine dye |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
dye–peptide conjugate |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/bios14100458 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2079-6374 |
| 研究者情報 |
|
|
URL |
https://hyokadb02.jimu.kyutech.ac.jp/html/332_ja.html |
| 研究者情報 |
|
|
URL |
https://hyokadb02.jimu.kyutech.ac.jp/html/326_ja.html |
| 論文ID（連携） |
|
|
値 |
10461663 |
| 連携ID |
|
|
値 |
14760 |
10461663.pdf (2.0 MB)
