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バイオセンシングおよびバイオイメージングアプリケーションのための近赤外高感度プローブの合理的設計
https://doi.org/10.18997/0002001909
https://doi.org/10.18997/00020019093b70647c-f7d0-47b1-aa14-c2d28aa89df6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
sei_k_510.pdf (4 MB)
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| Item type | 学位論文 = Thesis or Dissertation(1) | |||||||
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| 公開日 | 2025-08-25 | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| タイトル | ||||||||
| タイトル | Rational Design of Near -Infrared Sensitive Probes for Bio- sensing and Bio-imaging Applications | |||||||
| 言語 | en | |||||||
| タイトル | ||||||||
| タイトル | バイオセンシングおよびバイオイメージングアプリケーションのための近赤外高感度プローブの合理的設計 | |||||||
| 言語 | ja | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 著者 |
Priyanka,
× Priyanka,
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | This thesis presents the design, synthesis, and application of advanced squaraine-based fluorescent probes for enzyme detection and long-term in vivo bioimaging. By leveraging the unique photophysical properties of squaraine dyes, including their strong absorption in the near-infrared (NIR) region and tunable fluorescence behavior, this work addresses critical challenges in biological imaging and molecular sensing, such as sensitivity, specificity, and deep tissue penetration. The study begins with the development of squaraine dye-peptide conjugates tailored for the detection of trypsin, an enzyme with significant diagnostic and therapeutic implications. A novel dye-peptide probe (SQ-3 PC) was designed to exploit aggregation-induced quenching (AIQ) and Förster resonance energy transfer (FRET) mechanisms, enabling fluorescence ON/OFF sensing. This probe demonstrated exceptional sensitivity with a detection limit of 1.07 nM, providing a robust tool for enzyme-specific monitoring with potential applications in cancer diagnostics and proteomics research. It further extends to squaraine dyes with tunable optical properties for elastase detection. Structural modifications were employed to enhance their performance in aqueous environments, enabling precise and selective enzymatic sensing. The study highlights the adaptability of squaraine dyes to different enzymatic targets,showcasing their versatility and potential for broader diagnostic applications. In the context of bioimaging, the thesis explores the development of a squaraine dye-antibody conjugate for long-term NIR imaging. This conjugate exhibited superior photostability, deep tissue penetration, and minimal background interference, making it ideal for sustained in vivo imaging. These advancements position squaraine-based probes as powerful tools for visualizing dynamic biological processes, tracking disease progression, and monitoring therapeutic efficacy. Collectively, this work introduces a general and adaptable platform for designing advanced fluorescent probes that integrate AIQ, FRET, and NIR fluorescence. By addressing current limitations in biological imaging, these findings pave the way for the next generation of enzyme-specific biosensors and theranostic agents, advancing both fundamental research and translational applications in medicine. |
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| 目次 | ||||||||
| 内容記述タイプ | TableOfContents | |||||||
| 内容記述 | 1 INTRODUCTION| 2 INSTRUMENTATION AND CHARACTERIZATION| 3 SQUARAINE DYE-PEPTIDE CONJUGATE FOR SENSITIVE TRYPSIN DETECTION| 4 WAVELENGTH-TUNABLE SQUARAINE DYES AND THEIR APPLICATION IN ELASTASE DETECTION| 5 NIR SQUARAINE DYE-ANTIBODY CONJUGATE FOR LONGTERM IN VIVO FLUORESCENCE BIOIMAGING| 6 CONCLUSION AND FUTURE PROSPECTS |
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| 備考 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 九州⼯業⼤学博⼠学位論⽂ 学位記番号：生工博甲第510号 学位授与年⽉⽇: 令和7年3⽉25⽇ | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第510号 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（学術） | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2025-03-25 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 17104 | |||||||
| 言語 | ja | |||||||
| 学位授与機関名 | 九州工業大学 | |||||||
| 学位授与年度 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 令和6年度 | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| ID登録 | ||||||||
| ID登録 | 10.18997/0002001909 | |||||||
| ID登録タイプ | JaLC | |||||||
