{"created":"2023-05-15T11:56:45.857352+00:00","id":2121,"links":{},"metadata":{"_buckets":{"deposit":"b1e94a26-66db-4ed3-8be6-9cd4cdcfd853"},"_deposit":{"created_by":3,"id":"2121","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2121"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00002121","sets":["8:9"]},"author_link":["8055","8066","8062","8058","8054","8059","8053","8056","8063","8064","8065","8061"],"item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-07-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"12","bibliographicPageStart":"1","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Electronic Journal of Biotechnology"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens, and a probe for bacteria. In situ hybridization with methanogens and bacterial probes and denatured gradient gel electrophoresis within activated sludge clearly confirmed the presence of Methanosaeta sp. and Methanosarcina sp. cells. Methanosaeta concilii was found to be the dominant species in the bioreactor. These results revealed the presence of possibly new strain of Methanosaeta in the bioreactor for treating palm oil mill effluent called Methanosaeta concilii SamaliEB (Gene bank accession number: EU580025). In addition, fluorescent hybridization pictured the close association between the methanogens and bacteria and that the number of methanogens was greater than the number of bacteria.","subitem_description_type":"Abstract"}]},"item_21_description_60":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_21_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"affiliations":[{"affiliationNames":[{"affiliationName":"","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"Shirai","familyNameLang":"en"},{"familyName":"白井","familyNameLang":"ja"},{"familyName":"シライ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yoshihito","givenNameLang":"en"},{"givenName":"義人","givenNameLang":"ja"},{"givenName":"ヨシヒト","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8065","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50175395","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000050175395"},{"nameIdentifier":"7202788909","nameIdentifierScheme":"Scopus著者ID","nameIdentifierURI":"https://www.scopus.com/authid/detail.uri?authorId=7202788909"},{"nameIdentifier":"314","nameIdentifierScheme":"九工大研究者情報","nameIdentifierURI":"https://hyokadb02.jimu.kyutech.ac.jp/html/314_ja.html"}],"names":[{"name":"Shirai, Yoshihito","nameLang":"en"},{"name":"白井, 義人","nameLang":"ja"},{"name":"シライ, ヨシヒト","nameLang":"ja-Kana"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"Ikeno","familyNameLang":"en"},{"familyName":"池野","familyNameLang":"ja"},{"familyName":"イケノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Shinya","givenNameLang":"en"},{"givenName":"慎也","givenNameLang":"ja"},{"givenName":"シンヤ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"8066","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20437792","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000020437792"},{"nameIdentifier":"8646054000","nameIdentifierScheme":"Scopus著者ID","nameIdentifierURI":"https://www.scopus.com/authid/detail.uri?authorId=8646054000"},{"nameIdentifier":"331","nameIdentifierScheme":"九工大研究者情報","nameIdentifierURI":"https://hyokadb02.jimu.kyutech.ac.jp/html/331_ja.html"}],"names":[{"name":"Ikeno, Shinya","nameLang":"en"},{"name":"池野, 慎也","nameLang":"ja"},{"name":"イケノ, シンヤ","nameLang":"ja-Kana"}]}]},"item_21_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Pontificia Universidad Católica de Valparaíso"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2225/vol12-issue3-fulltext-4","subitem_relation_type_select":"DOI"}}]},"item_21_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2009 by Pontificia Universidad Católica de Valparaíso--Chili"}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0717-3458","subitem_source_identifier_type":"ISSN"}]},"item_21_text_36":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Bioprocess Technology Faculty of Biotechnology and Biomolecular Sciences University"},{"subitem_text_value":"Department of Bioprocess Technology Faculty of Biotechnology and Biomolecular Sciences University"},{"subitem_text_value":"Department of Cell and Molecular Biology Faculty of Biotech"}]},"item_21_text_64":{"attribute_name":"業績ID","attribute_value_mlt":[{"subitem_text_value":"439E55CF69253EE349257688003A91F9"}]},"item_21_version_type_58":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tabatabaei, Meisam"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zakaria, Rafein Mohd"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rahim, Abdul Raha"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wright, G. André-Denis"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Shirai, Yoshihito","creatorNameLang":"en"},{"creatorName":"白井, 義人","creatorNameLang":"ja"},{"creatorName":"シライ, ヨシヒト","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Abdullah, Norhani"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai, Kenji"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Ikeno, Shinya","creatorNameLang":"en"},{"creatorName":"池野, 慎也","creatorNameLang":"ja"},{"creatorName":"イケノ, シンヤ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Mori, Masatsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kazunori, Nakamura"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sulaiman, Alawi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hassan, Ali Mohd"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-12-21"}],"displaytype":"detail","filename":"11.PCR-based.pdf","filesize":[{"value":"732.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"11.PCR-based.pdf","url":"https://kyutech.repo.nii.ac.jp/record/2121/files/11.PCR-based.pdf"},"version_id":"e4fef06c-450b-46d0-8752-11d00d7f1f8e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"anaerobic digestion","subitem_subject_scheme":"Other"},{"subitem_subject":"DGGE","subitem_subject_scheme":"Other"},{"subitem_subject":"FISH","subitem_subject_scheme":"Other"},{"subitem_subject":"methanogens","subitem_subject_scheme":"Other"},{"subitem_subject":"POME","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent"}]},"item_type_id":"21","owner":"3","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-21"},"publish_date":"2009-12-21","publish_status":"0","recid":"2121","relation_version_is_last":true,"title":["PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-04-02T08:44:15.512463+00:00"}