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Mutation Analysis of the Interaction of B-type Cytochrome c Oxidase with its Natural Substrate Cytochrome c-551
http://hdl.handle.net/10228/4692
http://hdl.handle.net/10228/46926c7f608e-a870-47c5-80db-755ba5476eed
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
j.jbiosc.2009.10.008.pdf (222.1 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2010-07-13 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Mutation Analysis of the Interaction of B-type Cytochrome c Oxidase with its Natural Substrate Cytochrome c-551 | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Kabashima, Yoshiki
× Kabashima, Yoshiki× Ueda, Naoaki× Sone, Nobuhito× 坂本, 順司
WEKO
14076
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Heme-copper oxidases in the respiratory chain are classified into three subfamilies: A-, B- and C-types. Cytochrome bo3-type cytochrome c oxidase from thermophilic Bacillus is a B-type oxidase that is thought to interact with cytochrome c through hydrophobic interactions. This is in contrast to A-type oxidases, which bind cytochrome c molecules primarily through electrostatic forces between acidic residues in the oxidase subunit II and basic residues within cytochromes. In order to investigate the substrate-binding site in cytochrome bo3, eight acidic residues in subunit II were mutated to corresponding neutral residues and enzymatic activity was measured using cytochrome c-551 from closely related Bacillus PS3. The mutation of E116, located at the interface to subunit I, decreased the kcat value most prominently without affecting the Km value, indicating that the residue is important for electron transfer. The mutation of D99, located close to the CuA site, largely affected both values, suggesting that it is important for both electron transfer and substrate binding. The mutation of D49 and E84 did not affect enzyme kinetic parameters, but the mutation of E64, E66 and E68 lowered the affinity of cytochrome bo3 for cytochrome c-551 without affecting the kcat value. These three residues are located at the front of the hydrophilic globular domain and distant from the CuA site, suggesting that these amino acids compose an acidic patch for a second substrate-binding site. This is the first report on site-directed mutagenesis experiments of a B-type heme-copper oxidase. | |||||||||||||
| 書誌情報 |
Journal of Bioscience and Bioengineering 巻 109, 号 4, p. 325-330, 発行日 2010-04 |
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| 出版社 | ||||||||||||||
| 出版者 | 社団法人日本生物工学会 | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | info:doi/10.1016/j.jbiosc.2009.10.008 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1389-1723 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1347-4421 | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | Copyright (c) 2009 The Society for Biotechnology, Japan Published by Elsevier B.V. | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Geobacillus thermodenitrificans | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | B-type heme-copper oxidase | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | bo3-type cytochrome c oxidase | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Cytochrome c-551 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Thermophilic bacteria | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 研究者情報 | ||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/271_ja.html | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 35 | |||||||||||||
| 業績ID | ||||||||||||||
| 値 | 123466CD9853DAE549257704001CDF53 | |||||||||||||
| 情報源 | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.jbiosc.2009.10.008 | |||||||||||||
| 関連名称 | https://doi.org/10.1016/j.jbiosc.2009.10.008 | |||||||||||||
