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アイテム
遺伝子工学と生物化学的アプローチによるグリセロールを活用したバイオ燃料生産とバイオレメディエーション
https://doi.org/10.18997/00004380
https://doi.org/10.18997/0000438057f56319-59b2-44c4-b2a8-266fe3dfb41d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
sei_k_232.pdf (1.6 MB)
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| アイテムタイプ | 学位論文 = Thesis or Dissertation(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2016-03-23 | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| タイトル | ||||||||
| タイトル | Biofuels Production and Bioremediation using Glycerol by Genetic Engineering and Biochemical Approaches | |||||||
| 言語 | en | |||||||
| タイトル | ||||||||
| タイトル | 遺伝子工学と生物化学的アプローチによるグリセロールを活用したバイオ燃料生産とバイオレメディエーション | |||||||
| 言語 | ja | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 著者 |
Tran, Kien Trung
× Tran, Kien Trung
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Glycerol is the main byproduct from biodiesel production and expected to be a cheap feedstock for the production of useful substances. Furthermore, waste glycerol (WG) which is released from biodiesel production has a high pH level as well as contains toxic chemicals. Treatment of this waste requires a high cost, in turns raising cost to the biodiesel industry. Therefore, glycerol and WG should be either utilized or treated properly to make the biodiesel industry to be more competitive as well as to reduce the environmental burden. Here, in the framework of this thesis two approaches were taken: (1) Utilize glycerol as a feedstock for producing hydrogen via metabolic engineering in Escherichia coli; and (2) Bioremediation of WG and waste activated sludge (WAS) to produce biofuels (hydrogen and methane) and to reduce sludge.E. coli is a potential microorganism for hydrogen production because it can sustain a high growth rate, requires simple technology and could achieve a high hydrogen yield and production. However, hydrogen production from glycerol by E. coli is still not effective because of slow cell growth and low hydrogen production. Thus, we aimed to enhance hydrogen production from glycerol by using E. coli which can be easily manipulated. We took to sub-approaches: (i) improvement of glycerol metabolism toward hydrogen production by metabolic engineering; and (ii) random transposon mutagenesis to find beneficial knockouts for hydrogen production. In the first approach, a novel engineered strain with seven knockouts, BW25113 frdC ldhA fdnG ppc narG mgsA hycA was constructed and it shows 5-fold higher hydrogen productivity (μmol H2/ mg protein) than the parent strain. In the other approach, four genes, aroM, gatZ, ycgR, and yfgI were found by transposon mutagenesis and these knockouts showed better growth rate and hydrogen production.In the second approach, WAS which is released from the waste water treatment plant (WWTP), and is an environmental burden. Conventional treatment methods such as heating or dumping of WAS often require either high cost or large landfill, respectively. In practice, it is estimated that WAS treatment accounts for a half of the operating cost of the WWTP. By utilizing WAS as a microbial source to degrade glycerol and alkaline pH characteristic of WG to reduce WAS quantity, the anaerobic digestion was conduced with different WAS and WG ratios to figure out the optimal condition for sludge reduction and biofuel production. It is found that WAS was reduced by 62% under the condition of 25% WAS and 50% WG. Moreover, anaerobic digestion of 10% WAS and 1% WG was the optimal condition for producing hydrogen and methane. At this mode, hydrogen and methane production was increased by 24 and 8 fold in comparison to those of without supplementation of WG, respectively. Finally, codigestion of WAS and WG is economically viable for the WWTP, and this technique is more beneficial for the biodiesel fuels production manufactures. | |||||||
| 目次 | ||||||||
| 内容記述タイプ | TableOfContents | |||||||
| 内容記述 | 1. Introduction||2. Metabolic engineering of escherichia coli to enhance hydrogen production from glycerol||3. Identification of beneficial knockouts in escherichia coli for hydrogen production from glycerol||4. Waste activated sludge reduction and bio-fuels production from waste glycerol||5. General conclusions and future plan | |||||||
| 備考 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 九州工業大学博士学位論文 学位記番号:生工博甲第232号 学位授与年月日:平成27年3月25日 | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Glycerol | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Bio-hydrogen | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Escherichia coli | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Waste activated sludge | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Metabolic engineering | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Transposon mutagenesis | |||||||
| アドバイザー | ||||||||
| 前田, 憲成 | ||||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第232号 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（工学） | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2015-03-25 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 17104 | |||||||
| 学位授与機関名 | 九州工業大学 | |||||||
| 学位授与年度 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 平成26年度 | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| ID登録 | ||||||||
| ID登録 | 10.18997/00004380 | |||||||
| ID登録タイプ | JaLC | |||||||
