@article{oai:kyutech.repo.nii.ac.jp:00004711,
 author = {Kawashita, Masakazu and Hayashi, Jumpei and Kudo, Tada-aki and Kanetaka, Hiroyasu and Li, Zhixia and Miyazaki, Toshiki and 宮崎, 敏樹 and Hashimoto, Masami},
 issue = {6},
 journal = {Journal of biomedical materials research. Part A},
 month = {Jul},
 note = {Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.},
 pages = {1880--1886},
 title = {MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin},
 volume = {102},
 year = {2013},
 yomi = {ミヤザキ, トシキ}
}