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アイテム
Correlation Between Proton Translocation and Growth: Genetic Analysis of the Respiratory Chain of Corynebacterium glutamicum
http://hdl.handle.net/10228/00006148
http://hdl.handle.net/10228/000061481ca84691-2992-4e92-968b-08551a99ac7d
| 名前 / ファイル | ライセンス | アクション |
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JO146-6-845-855.pdf (578.2 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2017-04-18 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Correlation Between Proton Translocation and Growth: Genetic Analysis of the Respiratory Chain of Corynebacterium glutamicum | |||||||||||||
| 言語 | ja | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Kabashima, Yoshiki
× Kabashima, Yoshiki× Kishikawa, Jun-ichi× Kurokawa, Tatsuki× 坂本, 順司
WEKO
14076
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Corynebacterium glutamicum contains at least two terminal oxidases in the respiratory chain; cytochrome aa3-type cytochrome c oxidase and bd-type menaquinol oxidase. Thus, the chain has two branches of electron flow. The bcc-aa3 branch translocates three protons per electron transferred, while the bd branch translocates only one. In this study, we constructed two mutant strains, lacking of either subunit I of the cytochrome c oxidase (ΔctaD) or subunits I and II of the quinol oxidase (ΔcydAB), and also plasmids to complement the deficient genes to investigate their effects on energy conservation and cell growth. We measured H+/O ratios of C. glutamicum wild-type and mutant cells grown aerobically. The H+/O ratio of the wild-type cells grown in the semi-synthetic medium was 3.94 ± 0.30, while the value was 2.76 ± 0.25 for the ΔctaD mutant. In contrast, the value was 5.23 ± 0.36 for the ΔcydAB mutant. The cells grown in the LB medium showed higher value compared to that of cells grown in the semi-synthetic medium. The ΔctaD mutant grew less than the wild-type in LB medium, while they grew about equally in semi-synthetic medium. Correlation between bioenergetics and growth of C. glutamicum was significantly affected by the growth nutrients. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : The Journal of Biochemistry 巻 146, 号 6, p. 845-855, 発行日 2009-09-04 |
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| 出版社 | ||||||||||||||
| 出版者 | Oxford University Press | |||||||||||||
| 言語 | en | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1093/jb/mvp140 | |||||||||||||
| NCID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA00694073 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 1756-2651 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 0021-924X | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | Copyright (c) The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in The journal of biochemistry following peer review. The version of record J Biochem (2009) 146 (6): 845-855 is available online at: https://academic.oup.com/jb/article-abstract/146/6/845/809974/Correlation-Between-Proton-Translocation-and?redirectedFrom=fulltext and https://doi.org/10.1093/jb/mvp140. | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Corynebacterium glutamicum | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | cytochrome bd-type quinol oxidase | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | cytchrome aa3-type cytchrome c oxidase | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Gram-positive bacteria | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | H+/O ratio | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 研究者情報 | ||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/271_ja.html | |||||||||||||
| 論文ID（連携） | ||||||||||||||
| 値 | 10008463 | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 180 | |||||||||||||
