{"created":"2023-05-15T11:58:50.837757+00:00","id":5028,"links":{},"metadata":{"_buckets":{"deposit":"1a21fb89-e4f5-4d27-9894-a0a8ba57cafd"},"_deposit":{"created_by":3,"id":"5028","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5028"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00005028","sets":["8:9"]},"author_link":["6369","20148","20147","20151","20152","20149"],"item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1168","bibliographicPageStart":"1162","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"ACS biomaterials science & engineering"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Artificial hydroxyapatite (HAp) is osteoconductive, but the mechanism is still unclear. It is likely that some serum proteins are adsorbed onto HAp and influence its osteoconductivity. We investigated the adsorption behavior of laminin (LN), which was isolated from murine Engelbreth–Holm–Swarm sarcoma, onto HAp and compared it with nonosteoconductive alpha-type alumina (α-Al2O3). Cell adhesion, spreading, and proliferation on native and LN-adsorbed discs of HAp or α-Al2O3 were examined using murine MC3T3-E1 osteoblastic cells. A larger amount of LN adsorbed onto HAp than α-Al2O3 despite the electrostatic repulsion between LN and HAp, suggesting the specific adsorption of LN onto HAp. The LN adsorbed onto HAp remarkably enhanced initial attachment and spreading of MC3T3-E1 cells, but subsequent proliferation of MC3T3-E1 cells was influenced by the type of material rather than LN adsorption. These fundamental findings imply that LN adsorbed on HAp could trigger osteoconductivity in vivo, aiding in the development of novel biomaterials that specifically adsorb LN and effectively enhance cell attachment and spreading.","subitem_description_type":"Abstract"}]},"item_21_description_60":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_21_link_62":{"attribute_name":"研究者情報","attribute_value_mlt":[{"subitem_link_url":"https://hyokadb02.jimu.kyutech.ac.jp/html/323_ja.html"}]},"item_21_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Chemical Society"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acsbiomaterials.6b00190","subitem_relation_type_select":"DOI"}}]},"item_21_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"American Chemical Society"},{"subitem_rights":"This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in ACS biomaterials science & engineering, copyright © American Chemical Society after peer review. "},{"subitem_rights":"To access the final edited and published work, see http://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00190."}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12739394","subitem_source_identifier_type":"NCID"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2373-9878","subitem_source_identifier_type":"ISSN"}]},"item_21_text_28":{"attribute_name":"論文ID（連携）","attribute_value_mlt":[{"subitem_text_value":"10296953"}]},"item_21_text_36":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Biomedical Engineering, Tohoku University"},{"subitem_text_value":"Graduate School of Dentistry, Tohoku University"},{"subitem_text_value":"Graduate School of Dentistry, Tohoku University"},{"subitem_text_value":"Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology"},{"subitem_text_value":"Japan Fine Ceramics Center"},{"subitem_text_value":"Graduate School of Biomedical Engineering, Tohoku University"}]},"item_21_text_63":{"attribute_name":"連携ID","attribute_value_mlt":[{"subitem_text_value":"5470"}]},"item_21_version_type_58":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujita,  Hiroka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kudo,  Tada-aki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanetaka,  Hiroyasu"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Miyazaki, Toshiki","creatorNameLang":"en"},{"creatorName":"宮崎, 敏樹","creatorNameLang":"ja"},{"creatorName":"ミヤザキ, トシキ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{},{}]},{"creatorNames":[{"creatorName":"Hashimoto,  Masami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawashita,  Masakazu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-22"}],"displaytype":"detail","filename":"ACSbse2_1162.pdf","filesize":[{"value":"514.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ACSbse2_1162.pdf","url":"https://kyutech.repo.nii.ac.jp/record/5028/files/ACSbse2_1162.pdf"},"version_id":"3a1aee0c-bcfe-4479-b4f7-293d6a9d2c68"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"adsorption","subitem_subject_scheme":"Other"},{"subitem_subject":"bioactivity","subitem_subject_scheme":"Other"},{"subitem_subject":"hydroxyapatite","subitem_subject_scheme":"Other"},{"subitem_subject":"laminin; MC3T3-E1","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoconductivity","subitem_subject_scheme":"Other"},{"subitem_subject":"α-alumina","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Adsorption of Laminin on Hydroxyapatite and Alumina and the MC3T3-E1 Cell Response","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Adsorption of Laminin on Hydroxyapatite and Alumina and the MC3T3-E1 Cell Response"}]},"item_type_id":"21","owner":"3","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-06-22"},"publish_date":"2017-06-22","publish_status":"0","recid":"5028","relation_version_is_last":true,"title":["Adsorption of Laminin on Hydroxyapatite and Alumina and the MC3T3-E1 Cell Response"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-04-02T08:32:54.567633+00:00"}