@article{oai:kyutech.repo.nii.ac.jp:00005162,
 author = {Liu,  Zhongmei and Takazaki,  Hiroko and Nakazawa,  Yuki and Sakato,  Miho and Yagi,  Toshiki and Yasunaga, Takuo and 安永, 卓生 and King,  Stephen M. and Kamiya,  Ritsu},
 issue = {7},
 journal = {Eukaryotic Cell},
 month = {May},
 note = {The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest. Here we report the isolation of a novel mutant, oda2-t, whose γ heavy chain is truncated at about 30% of the sequence. While the previously isolated γ chain mutant oda2 lacks the entire outer arm, oda2-t retains outer arms that contain α and β heavy chains, suggesting that the N-terminal sequence (corresponding to the tail region) is necessary and sufficient for stable outer-arm assembly. Thin-section electron microscopy and image analysis localize the γ heavy chain to a basal region of the outer-arm image in the axonemal cross section. The motility of oda2-t is lower than that of the wild type and oda11 (lacking the α heavy chain) but higher than that of oda2 and oda4-s7 (lacking the motor domain of the β heavy chain). Thus, the outer-arm dynein lacking the γ heavy-chain motor domain is partially functional. The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein.},
 pages = {1136--1145},
 title = {Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain},
 volume = {7},
 year = {2008},
 yomi = {ヤスナガ, タクオ}
}