{"created":"2023-05-15T11:58:56.537340+00:00","id":5162,"links":{},"metadata":{"_buckets":{"deposit":"e4a4e98f-76b3-40b1-be3f-fce7635a601c"},"_deposit":{"created_by":3,"id":"5162","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5162"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00005162","sets":["8:9"]},"author_link":["7216","20727","20724","20723","20725","20722","20728","20721"],"control_number":"5162","item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-05-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1145","bibliographicPageStart":"1136","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Eukaryotic Cell"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest. Here we report the isolation of a novel mutant, oda2-t, whose γ heavy chain is truncated at about 30% of the sequence. While the previously isolated γ chain mutant oda2 lacks the entire outer arm, oda2-t retains outer arms that contain α and β heavy chains, suggesting that the N-terminal sequence (corresponding to the tail region) is necessary and sufficient for stable outer-arm assembly. Thin-section electron microscopy and image analysis localize the γ heavy chain to a basal region of the outer-arm image in the axonemal cross section. The motility of oda2-t is lower than that of the wild type and oda11 (lacking the α heavy chain) but higher than that of oda2 and oda4-s7 (lacking the motor domain of the β heavy chain). Thus, the outer-arm dynein lacking the γ heavy-chain motor domain is partially functional. The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein.","subitem_description_type":"Abstract"}]},"item_21_description_60":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_21_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1128/EC.00102-08","subitem_relation_type_select":"DOI"}}]},"item_21_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://ec.asm.org/cgi/content/abstract/7/7/1136","subitem_relation_type_select":"URI"}}]},"item_21_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"American Society for Microbiology"}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1535-9778","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"1535-9786","subitem_source_identifier_type":"EISSN"}]},"item_21_text_36":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biological Sciences, Graduate School of Science, University of Tokyo"},{"subitem_text_value":"Department of Bioscience and Bioinformatics, Kyushu Institute of Technology"},{"subitem_text_value":"Department of Biological Sciences, Graduate School of Science, University of Tokyo"},{"subitem_text_value":"Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center"},{"subitem_text_value":"Department of Biological Sciences, Graduate School of Science, University of Tokyo/ Structural Biology, Graduate School of Science, Kyoto University"},{"subitem_text_value":"Department of Bioscience and Bioinformatics, Kyushu Institute of Technology"},{"subitem_text_value":"Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center"},{"subitem_text_value":"Department of Biological Sciences, Graduate School of Science, University of Tokyo/ CREST, Japan Science and Technology Corporation"}]},"item_21_text_63":{"attribute_name":"連携ID","attribute_value_mlt":[{"subitem_text_value":"643"}]},"item_21_version_type_58":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Liu,  Zhongmei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takazaki,  Hiroko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakazawa,  Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakato,  Miho","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yagi,  Toshiki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Yasunaga, Takuo","creatorNameLang":"en"},{"creatorName":"安永, 卓生","creatorNameLang":"ja"},{"creatorName":"ヤスナガ, タクオ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{},{},{}]},{"creatorNames":[{"creatorName":"King,  Stephen M.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamiya,  Ritsu","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-13"}],"displaytype":"detail","filename":"Eukaryotic Cell-2008-Liu-1136-45.pdf","filesize":[{"value":"776.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Eukaryotic Cell-2008-Liu-1136-45.pdf","url":"https://kyutech.repo.nii.ac.jp/record/5162/files/Eukaryotic Cell-2008-Liu-1136-45.pdf"},"version_id":"7e5cd1f1-4054-48c6-8f2e-c94c529b29b3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain","subitem_title_language":"en"}]},"item_type_id":"21","owner":"3","path":["9"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-13"},"publish_date":"2017-09-13","publish_status":"0","recid":"5162","relation_version_is_last":true,"title":["Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-02T08:33:49.827567+00:00"}