{"created":"2023-05-15T11:58:58.100459+00:00","id":5199,"links":{},"metadata":{"_buckets":{"deposit":"96021f0f-292a-4e72-91cd-8e323829d986"},"_deposit":{"created_by":3,"id":"5199","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5199"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00005199","sets":["8:9"]},"author_link":["8066","20926","20925"],"item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"342","bibliographicPageStart":"337","bibliographicVolumeNumber":"134","bibliographic_titles":[{"bibliographic_title":"Analyst"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We propose that the most sophisticated strategy for primary biosurveillance is to exploit structural commonality through the detection of biologically relevant phosphoric substances. A novel assay, an artificial-enzyme membrane was designed and synthesized for sensor fabrication. This artificial-enzyme catalyzes the hydrolysis of the diphosphoric acid anhydride structure. This structure-selective, albeit not molecule-selective, catalytic hydrolysis was successfully coupled with amperometric detection. Since the catalytic reaction produces a dephosphorylation product (PO43−), it can be reduced by an electrode potential of −250 mV vs. Ag/AgCl. Owing to the structural selectivity of the artificial-enzyme membrane, the sensor can detect biological phosphoric substances comprehensively that have the diphosphoric acid anhydride structure. The sensor successfully determined various biological phosphoric substances at concentrations in the micromolar (µM) to millimolar (mM) range, and it showed good functional stability and reproducibility in terms of sensor responses. This sensor was used to detect Escherichia coli lysed by heat treatment, and the response increased with increasing bacterial numbers. This unique technique for analyzing molecular commonality can be applied to the surveillance of biocontaminants, e.g. microorganisms, spores and viruses. Artificial-enzyme-based detection is a novel strategy for practical biosurveillance in the front line.","subitem_description_type":"Abstract"}]},"item_21_description_60":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_21_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Royal Society of Chemistry"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1039/B810846C","subitem_relation_type_select":"DOI"}}]},"item_21_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.rsc.org/publishing/journals/AN/article.asp?doi=b810846c","subitem_relation_type_select":"URI"}}]},"item_21_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Royal Society of Chemistry"}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0003-2654","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1364-5528","subitem_source_identifier_type":"ISSN"}]},"item_21_text_36":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biological functions and Engineering, Kyushu Institute of Technology/CREST, Japan Science and Technology Agency"},{"subitem_text_value":"Department of Biological functions and Engineering, Kyushu Institute of Technology"},{"subitem_text_value":"Department of Biological functions and Engineering, Kyushu Institute of Technology/CREST, Japan Science and Technology Agency"}]},"item_21_text_63":{"attribute_name":"連携ID","attribute_value_mlt":[{"subitem_text_value":"625"}]},"item_21_version_type_58":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Ikeno, Shinya","creatorNameLang":"en"},{"creatorName":"池野, 慎也","creatorNameLang":"ja"},{"creatorName":"イケノ, シンヤ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yoshida,  Tetsuya"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Haruyama, Tetsuya","creatorNameLang":"en"},{"creatorName":"春山, 哲也","creatorNameLang":"ja"},{"creatorName":"ハルヤマ, テツヤ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-17"}],"displaytype":"detail","filename":"an_134_2.pdf","filesize":[{"value":"611.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"an_134_2.pdf","url":"https://kyutech.repo.nii.ac.jp/record/5199/files/an_134_2.pdf"},"version_id":"a2f095ac-d541-457b-bb8e-08518e194a2a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-17"}],"displaytype":"detail","filename":"an_134_2_references.pdf","filesize":[{"value":"20.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"an_134_2_references.pdf","url":"https://kyutech.repo.nii.ac.jp/record/5199/files/an_134_2_references.pdf"},"version_id":"69d7a186-9b63-41ee-9080-a3ac35959331"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"artificial-enzyme membrane","subitem_subject_scheme":"Other"},{"subitem_subject":"bio-surveillance","subitem_subject_scheme":"Other"},{"subitem_subject":"dephosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"electrochemical detection","subitem_subject_scheme":"Other"},{"subitem_subject":"PO43-","subitem_subject_scheme":"Other"},{"subitem_subject":"poly acrylamide","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Artificial-enzyme gel membrane-based biosurveillance sensor with high reproducibility and long-term storage stability","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Artificial-enzyme gel membrane-based biosurveillance sensor with high reproducibility and long-term storage stability"}]},"item_type_id":"21","owner":"3","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-17"},"publish_date":"2017-10-17","publish_status":"0","recid":"5199","relation_version_is_last":true,"title":["Artificial-enzyme gel membrane-based biosurveillance sensor with high reproducibility and long-term storage stability"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-04-02T08:42:33.316964+00:00"}