WEKO3
アイテム
Computer-aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli
http://hdl.handle.net/10228/00006441
http://hdl.handle.net/10228/000064413c4d59ae-c926-44ae-a60b-07a760927baa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
msb_4_160.pdf (393.5 kB)
|
.png)
|
| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2017-11-29 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Computer-aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Nishio, Yousuke
× Nishio, Yousuke× Usuda, Yoshihiro× Matsui, Kazuhiko× 倉田, 博之
WEKO
2130
|
|||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The phosphotransferase system (PTS) is the sugar transportation machinery that is widely distributed in prokaryotes and is critical for enhanced production of useful metabolites. To increase the glucose uptake rate, we propose a rational strategy for designing the molecular architecture of the Escherichia coli glucose PTS by using a computer-aided design (CAD) system and verified the simulated results with biological experiments. CAD supports construction of a biochemical map, mathematical modeling, simulation, and system analysis. Assuming that the PTS aims at controlling the glucose uptake rate, the PTS was decomposed into hierarchical modules, functional and flux modules, and the effect of changes in gene expression on the glucose uptake rate was simulated to make a rational strategy of how the gene regulatory network is engineered. Such design and analysis predicted that the mlc knockout mutant with ptsI gene overexpression would greatly increase the specific glucose uptake rate. By using biological experiments, we validated the prediction and the presented strategy, thereby enhancing the specific glucose uptake rate. | |||||||||||||
| 書誌情報 |
Molecular Systems Biology 巻 4, 号 1, p. 160, 発行日 2008-01-15 |
|||||||||||||
| 出版社 | ||||||||||||||
| 出版者 | John Wiley & Sons, Inc. | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | info:doi/10.1038/msb4100201 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1744-4292 | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | Copyright (c) 2008 EMBO and Nature Publishing Group | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Escherichia coli | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | glucose uptake | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | phosphotransferase system | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | regulation; system analysis | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 研究者情報 | ||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/265_ja.html | |||||||||||||
| 論文ID（連携） | ||||||||||||||
| 値 | 10028791 | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 312 | |||||||||||||
| 情報源 | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1038/msb4100201 | |||||||||||||
| 関連名称 | https://doi.org/10.1038/msb4100201 | |||||||||||||
