{"created":"2023-05-15T11:59:04.199874+00:00","id":5335,"links":{},"metadata":{"_buckets":{"deposit":"e5856991-d96a-4fc5-aad4-b1a7a3c9bf9e"},"_deposit":{"created_by":18,"id":"5335","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"5335"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00005335","sets":["6:7"]},"author_link":[],"item_20_date_granted_61":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-12-27"}]},"item_20_degree_grantor_59":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"九州工業大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17104","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_20_degree_name_58":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（工学）"}]},"item_20_description_30":{"attribute_name":"目次","attribute_value_mlt":[{"subitem_description":"1 Introduction and literature review||2 General materials and methods||3 Bacterial soldiers in waste activated sludge – understanding of the bacterial natural selection for the future successful bioremediation –||4 Impact of different antibiotics on methane production using waste-activated sludge: mechanisms and microbial community dynamics||5 Effect of macrolides and lincosamides on methane production using waste activated sludge: mechanisms and microbial community dynamics||6 Inhibition of a mimic enteric methane fermentation by a palm oil industrial waste, phospholine gum – understanding of microbial community dynamics –||7 Concluding remarks and suggestions for further research","subitem_description_type":"TableOfContents"}]},"item_20_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Waste activated sludge （WAS） is a by-product that has been abundantly produced from wastewater treatment plant after secondary biological treatment. The disposal of WAS through landfill or incineration has created more vulnerable problems in term of cost and environmental issues. Many strategies have adapted bioremediation and bioenergy production in order to completely utilize this WAS for sustainable environment and biodiversity conservation. These processes are driven by a complex microorganisms in WAS. Therefore, the successful achievement of both strategies; bioremediation and bioenergy production can be clarified based on the microbial community profile through next-generation sequencing analysis, MiSeq. Bioremediation strategies utilize specific and powerful exogenous microorganisms to assist in the degradation of various harmful pollutants in WAS. However, this inoculant is unable to retain in WAS for a long period of time to carry out their specific functions in degradation. The interaction between this inoculant and indigenous microbes presence in WAS is suspected to be one of the main reasons. Therefore, through the microbial community analysis in this study, some indigenous bacteria have been identified as bacterial soldiers that are responsible in killing the inoculant bacteria. The enhanced green fluorescence protein （EGFP） expressing E. coli was used as the inoculant with the fluorescence marker and some antibiotic-resistant genes. The indigenous bacteria identified as bacterial soldiers were mainly from Comamonadaceae, Myxococcales and Sphingobacteriales communities as later proved by co-culture interaction with inoculant.  Bioenergy production through methane fermentation is another approach that has been taken to utilize WAS. Many studies have been done in laboratory scale to find an efficient methane production approach including application of antibiotic, azithromycin. The effect of antibiotics on microbial diversity during anaerobic digestion stages is important to understand their mechanisms and functions in methane production. Therefore, in this study, different antibiotics that produced different methane profile were used to clarify the microbial interactions and regulatory systems in each stages of anaerobic digestion process. During anaerobic digestion, hydrolysis and fermentation stages were efficiently occurred due to the activation of hydrolytic and fermentative bacterial communities by all antibiotics used in this study. However, some antibiotics have shaped the unfavorable conditions for methanogens in methanogenesis stages. This unbalanced of microbial communities has affected on methane production. Thus, a balanced of microbial communities in all stages of anaerobic digestion is important for efficient methane production as shown by some antibiotics, including azithromycin. The importance of microbial communities was shown in bioremediation and methane fermentation processes. Another application using by-product taken from refining process of palm oil industry, phospholine gum was done for methane fermentation in WAS. It was found that phospholine gum has inhibited methane production but not affected other anaerobic digestion stages. Therefore, based on these phenomena, phospholine gum has potential to be applied as feed additive for ruminants in mitigating the enteric methane emission which contribute to greenhouse gases.  In conclusion, various reflections and dynamics have been shown by microbial diversity due to the different effects and conditions in the biological processes. This has provided a better and clear understanding on their functions and mechanisms in ensuring a successful bioremediation process and bioenergy production as the excellent approaches for sustainable environment and biodiversity conservation.","subitem_description_type":"Abstract"}]},"item_20_description_5":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_description":"九州工業大学博士学位論文 学位記番号：生工博甲第275号 学位授与年月日：平成28年12月27日","subitem_description_type":"Other"}]},"item_20_description_60":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"平成28年度","subitem_description_type":"Other"}]},"item_20_dissertation_number_62":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第275号"}]},"item_20_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18997/00006546","subitem_identifier_reg_type":"JaLC"}]},"item_20_text_34":{"attribute_name":"アドバイザー","attribute_value_mlt":[{"subitem_text_value":"白井, 義人"}]},"item_20_version_type_63":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nurul,  Asyifah Mustapha","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-05"}],"displaytype":"detail","filename":"sei_k_275.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"sei_k_275.pdf","objectType":"fulltext","url":"https://kyutech.repo.nii.ac.jp/record/5335/files/sei_k_275.pdf"},"version_id":"bd501495-72e3-4325-9169-09812eb87911"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Microbial community","subitem_subject_scheme":"Other"},{"subitem_subject":"Waste activated sludge","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacterial soldiers","subitem_subject_scheme":"Other"},{"subitem_subject":"Methane","subitem_subject_scheme":"Other"},{"subitem_subject":"Antibiotics","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Microbial Community Dynamics to Understand Bacterial Interaction for Bioremediation and Bioenergy Production","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Microbial Community Dynamics to Understand Bacterial Interaction for Bioremediation and Bioenergy Production","subitem_title_language":"en"},{"subitem_title":"バイオレメディエーションとバイオエネルギー生産のための細菌間相互作用を理解するための微生物群集動態解析","subitem_title_language":"ja"}]},"item_type_id":"20","owner":"18","path":["7"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-02-05"},"publish_date":"2018-02-05","publish_status":"0","recid":"5335","relation_version_is_last":true,"title":["Microbial Community Dynamics to Understand Bacterial Interaction for Bioremediation and Bioenergy Production"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-16T01:53:13.607858+00:00"}