{"created":"2023-05-15T11:59:06.001971+00:00","id":5378,"links":{},"metadata":{"_buckets":{"deposit":"ab2a4225-bb3e-4e4a-a51e-17f0406f5678"},"_deposit":{"created_by":3,"id":"5378","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5378"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00005378","sets":["8:24"]},"author_link":["21661","21663","21657","21659","21654","21656","21660","21662","21658"],"item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-08-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"e104695-10","bibliographicPageStart":"e104695-1","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLoS ONE "}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this work, lipase from Candida rugosa was immobilized onto chitosan/graphene oxide beads. This was to provide an enzyme-immobilizing carrier with excellent enzyme immobilization activity for an enzyme group requiring hydrophilicity on the immobilizing carrier. In addition, this work involved a process for the preparation of an enzymatically active product insoluble in a reaction medium consisting of lauric acid and oleyl alcohol as reactants and hexane as a solvent. This product enabled the stability of the enzyme under the working conditions and allowed the enzyme to be readily isolated from the support. In particular, this meant that an enzymatic reaction could be stopped by the simple mechanical separation of the “insoluble” enzyme from the reaction medium. Chitosan was incorporated with graphene oxide because the latter was able to enhance the physical strength of the chitosan beads by its superior mechanical integrity and low thermal conductivity. The X-ray diffraction pattern showed that the graphene oxide was successfully embedded within the structure of the chitosan. Further, the lipase incorporation on the beads was confirmed by a thermo-gravimetric analysis. The lipase immobilization on the beads involved the functionalization with coupling agents, N-hydroxysulfosuccinimide sodium (NHS) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC), and it possessed a high enzyme activity of 64 U. The overall esterification conversion of the prepared product was 78% at 60°C, and it attained conversions of 98% and 88% with commercially available lipozyme and novozyme, respectively, under similar experimental conditions.","subitem_description_type":"Abstract"}]},"item_21_description_60":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_21_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1371/journal.pone.0104695","subitem_relation_type_select":"DOI"}}]},"item_21_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2014 Lau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited"}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_21_text_36":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia"},{"subitem_text_value":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, Institute of Advanced Technology, University Putra Malaysia, Serdang, Selangor, Malaysia"},{"subitem_text_value":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia"},{"subitem_text_value":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang"},{"subitem_text_value":"Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia"},{"subitem_text_value":"Low Dimensional Materials Research Centre, Department of Physics, Faculty of Science, University of Malaya"},{"subitem_text_value":"Low Dimensional Materials Research Centre, Department of Physics, Faculty of Science, University of Malaya"},{"subitem_text_value":"School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia"},{"subitem_text_value":"Eco-Town Collaborative Research and Development Center for the Environment and Recycling, Kyushu Institute of Technology"}]},"item_21_text_63":{"attribute_name":"連携ID","attribute_value_mlt":[{"subitem_text_value":"6679"}]},"item_21_version_type_58":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lau,  Siaw Cheng"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lim ,  Hong Ngee"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Basri,  Mahiran"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masoumi,  Hamid Reza Fard"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tajudin,  Asilah Ahmad"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Huang,  Nay Ming"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Pandikumar,  Alagarsamy"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chia,  Chi Hua"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Ando, Yoshito","creatorNameLang":"en"},{"creatorName":"安藤, 義人","creatorNameLang":"ja"},{"creatorName":"アンドウ, ヨシト","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-28"}],"displaytype":"detail","filename":"pone9_e104695.PDF","filesize":[{"value":"8.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"pone9_e104695.PDF","url":"https://kyutech.repo.nii.ac.jp/record/5378/files/pone9_e104695.PDF"},"version_id":"215f4e65-80dd-402d-8a6e-e13b60ea81d4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads"}]},"item_type_id":"21","owner":"3","path":["24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-28"},"publish_date":"2018-02-28","publish_status":"0","recid":"5378","relation_version_is_last":true,"title":["Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-10-26T01:14:47.736493+00:00"}