WEKO3
アイテム
Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172
http://hdl.handle.net/10228/00006594
http://hdl.handle.net/10228/00006594ed25022d-ee44-4db5-8ab5-00d2d496e4ed
| 名前 / ファイル | ライセンス | アクション |
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JMBT4_103.pdf (1.7 MB)
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| Item type | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2018-02-28 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172 | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
| 著者 |
Yee, Lian-Ngit
× Yee, Lian-Ngit× Mumtaz, Tabassum× Mohammadi, Mitra× Phang, Lai-Yee× 安藤, 義人
WEKO
21654
× Rahim Raha, Abdul× Sudesh, Kumar× Ariffin, Hidayah× Hassan, Mohd Ali× Zakaria, Mohd Rafein |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Recombinant Escherichia coli JM109 harbouring the polyhydroxyalkanoate (PHA) biosynthesis gene (phaCABco) of Comamonas sp. EB172, an acid tolerant microbe, was examined for the production of PHAs from various carbon sources. The study demonstrated that the recombinant E. coli JM109 had the potential to utilize both sugar- and acid-based carbon sources, for the biosynthesis of both poly(3-hydroxybutyrate) P(3HB) and poly(3- hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymers. In the shake flask experiments, the strain was capable of producing P (3HB-co-3HV) copolymer from mixed organic acids, and higher productivities were obtained using glucose compared to mixed acids. However, PHA accumulation was found to be similar, regardless of the carbon source used. Nitrogen supplementation in the medium was found to improve the cell dry weight, but negatively affected the 3HV formation in copolymer production. Maximum 3HV monomer (3 mol%) was obtained with C/N 42.1, using mixed acids as the carbon source. In the 2L bioreactor, the productivity and yield based on substrate utilization coefficient were found to be 0.16 g PHA/(L.h) and 0.41 g PHA/g substrate under C/N around 75, using 20 g/L glucose and 0.5 g/L ammonium sulphate, respectively. The polymer produced by the recombinant strain had molecular weight in the range of 8.5 x 105 to 1.4 x 106 Da. Overall, the ability of the recombinant E. coli JM109 to utilize both glucose and mixed acids, has widened its substrate selection for fermentation, including the opportunity to use renewable biomass | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Journal of Microbial and Biochemical Technology 巻 4, 号 4, p. 103-110, 発行日 2012-09-25 |
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| 出版社 | ||||||||||||||
| 出版者 | OMICS International | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.4172/1948-5948.1000079 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 1948-5948 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Recombinant | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | PhaCABco | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Mixed acids | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Polyhydroxyalkanoate | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Comamonas sp. EB172 | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 6682 | |||||||||||||
