WEKO3
アイテム
Comparative study of methods for extraction and purification of environmental DNA from high-strength wastewater sludge
http://hdl.handle.net/10228/00006611
http://hdl.handle.net/10228/000066116393169d-de92-493e-97df-215e2d02dea5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AJB9_4926.pdf (349.7 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2018-03-01 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Comparative study of methods for extraction and purification of environmental DNA from high-strength wastewater sludge | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Tabatabaei, Meisam
× Tabatabaei, Meisam× Zakaria, Mohd Rafein× Rahim, Raha Abdul× Abdullah, Norhani× Wright, andré-Denis G.× 白井, 義人
WEKO
8065
× Shamsara, Mehdi× Sakai, Kenji× Hassan, Mohd Ali |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | DNA extraction from wastewater sludge (COD 50000 and BOD 25000 mg/l) was conducted using nine different methods normally used for environmental samples including a procedure used in this study and the results obtained were compared. The quality of the differently extracted DNAs was subsequently assessed by measuring humic acid concentration, cell lysis efficiency, polymerase chain reaction (PCR) amplification of methanogenic and eubacterial 16S rDNA. The protocol developed in this study was further evaluated by extracting DNA from various high-strength wastewater sludge samples, denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) analyses. The results revealed that great differences existed among the nine procedures and only a few produced satisfactory results when applied to high-strength wastewater sludge. Thermal shock alone was shown inefficient to disrupt the methanogenic cell wall to release the DNA. The method presented in this study (Procedure 9) is generally recommended because of the low concentration of contaminants and its high efficiency despite its simplicity. | |||||||||||||
| 書誌情報 |
African Journal of Biotechnology 巻 9, 号 31, p. 4926-4937, 発行日 2010-08-02 |
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| 出版社 | ||||||||||||||
| 出版者 | Academic Journals | |||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 1684-5315 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | High-strength wastewater sludge | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | DNA extraction | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | environmental samples | |||||||||||||
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| 主題Scheme | Other | |||||||||||||
| 主題 | humic acids | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | denaturing gradient gel electrophoresis | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | fluorescent in situhybridization | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 6700 | |||||||||||||
| 情報源 | ||||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://www.academicjournals.org/journal/AJB | |||||||||||||
