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Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost
http://hdl.handle.net/10228/00006620
http://hdl.handle.net/10228/00006620eab6db3e-8f75-457a-b8bd-09e07bbf318a
| 名前 / ファイル | ライセンス | アクション |
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AJAS7_56.pdf (318.3 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||
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| 公開日 | 2018-03-02 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| タイトル | ||||||
| タイトル | Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 著者 |
Baharuddin, Azhari Samsu
× Baharuddin, Azhari Samsu× Razak, Mohamad Nafis Abd× Hock, Lim Siong× Ahmad, Mohd Najib× Abd-Aziz, Suraini× Rahman, Nor' Aini Abdul× Shah, Umi Kalsom Md× Hassan, Mohd Ali× Sakai, Kenji× 白井, 義人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% lignin content. The temperature, pH and moisture content of the composting pile at day 7 treatment were 58.3, 8.1 and 65.5°C, respectively. The morphological analysis of the isolated microbes was conducted using Scanning Electron Microscope (SEM) and Gram stain method. The congo red test was conducted in order to detect 1% CMC agar degradation activities. Total genomic DNAs were extracted from approximately 1.0 g of mixed compost and amplified by using PCR primers. The PCR product was sequent to identify the nearest relatives of 16S rRNA genes. The localization of bacteria chromosomes was determined by Fluorescence In Situ Hybridization (FISH) analysis. Results: Single isolated bacteria species was successfully isolated from Empty Fruit Bunch (EFB)-Palm Oil Mill Effluent (POME) compost at thermophilic stage. Restriction fragment length polymorphism profiles of the DNAs coding for the 16S rRNAs with the phylogenetic analysis showed that the isolated bacteria from EFB-POME thermophilic compost gave the highest homology (99%) with similarity to Geobacillus pallidus. The strain was spore forming bacteria and able to grow at 60°C with pH 7. Conclusion: Thermophilic bacteria strain, Geobacillus pallidus was successfully isolated from Empty Fruit Bunch (EFB) and Palm Oil Mil Effluent (POME) compost and characterized. |
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| 言語 | en | |||||
| 書誌情報 |
en : American Journal of Applied Sciences 巻 7, 号 1, p. 56-62, 発行日 2010-01-31 |
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| 出版社 | ||||||
| 出版者 | Science Publications | |||||
| 言語 | en | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3844/ajassp.2010.56.62 | |||||
| URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://thescipub.com/journals/ajas/ | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12073324 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 1554-3641 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1546-9239 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cellulase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | thermophilic bacteria | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | composting | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | empty fruit bunch | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | palm oil mill effluent | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 査読の有無 | ||||||
| 値 | yes | |||||
| 連携ID | ||||||
| 値 | 6705 | |||||
