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Evaluation of Factors Affecting Polyhydroxyalkanoates Production by Comamonas sp. EB172 Using Central Composite Design
http://hdl.handle.net/10228/00006633
http://hdl.handle.net/10228/00006633cb8ba87d-df92-466f-9e39-920cac11229b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
MJM8_184.pdf (374.1 kB)
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| Item type | 学術雑誌論文 = Journal Article(1) | |||||
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| 公開日 | 2018-03-06 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| タイトル | ||||||
| タイトル | Evaluation of Factors Affecting Polyhydroxyalkanoates Production by Comamonas sp. EB172 Using Central Composite Design | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 著者 |
Johar, Noor Azman Mohd
× Johar, Noor Azman Mohd× Hassan, Mohd Ali× Zakaria, Mohd Rafein× Yee, Phang Lai× 白井, 義人× Ariffin, Hidayah |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Aims: Statistical approach, central composite design (CCD) was used to investigate the complex interaction among temperature (25-37℃), initial medium pH (5-9), inoculum size (4-10 % (v/v)), concentration of (NH4)2SO4 (0-1 g/L) and concentration of mixed organic acids (5-10 g/L) in the production of polyhydroxyalkanoates by Comamonas sp. EB172. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Methodology and Results: Mixed organic acids derived from anaerobically treated palm oil mill effluent (POME) containing acetic:propionic:butyric (ratio of 3:1:1) were used as carbon source in the batch culture of Comamonas sp. EB172 to produce polyhydoxyalkanoates (PHAs). The analysis of variance (ANOVA) showed that all five factors were significantly important in the batch fermentation by shake flask with a P value of less than 0.001. The optimal temperature, initial medium pH, inoculum size, concentration of (NH4)2SO4 and concentration of mixed organic acids were 30℃, 7.04, 4.0 % (v/v), 0.01 g/L and 5.05 g/L respectively. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Conclusion, significance and impact of study: Optimization of the production medium containing mixed organic acids has improved the PHA production for more than 2 folds. Under optimal condition in the shake flask fermentation, the predicted growth is 2.98 g/L of dry cell weight (DCW) with 47.07 wt % of PHA content. The highest yield of PHA was 0.28 g of PHA per g mixed organic acids. | |||||
| 書誌情報 |
Malaysian Journal of Microbiology 巻 8, 号 3, p. 184-190, 発行日 2012-09 |
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| 出版社 | ||||||
| 出版者 | Malaysian Society for Microbiology | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.21161/mjm.04612 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1823-8262 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2231-7538 | |||||
| 著作権関連情報 | ||||||
| 権利情報 | Malaysian Society for Microbiology | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | optimization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | central composite design | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Comamonas sp. EB172 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | polyhydroxyalkanoate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | response surface | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | methodology | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 査読の有無 | ||||||
| 値 | yes | |||||
| 連携ID | ||||||
| 値 | 6717 | |||||
