WEKO3
アイテム
Production and characterization of cellulases by Bacillus pumilus EB3
http://hdl.handle.net/10228/00006649
http://hdl.handle.net/10228/000066493ea58bec-7ce2-4301-92d7-143ee567ef24
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
IJEE3_47.pdf (308.7 kB)
|
|
| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2018-03-14 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Production and characterization of cellulases by Bacillus pumilus EB3 | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Ariffin, Hidayah
× Ariffin, Hidayah× Abdullah, Norhafizah× Md Shah, Umi Kalsom× 白井, 義人
WEKO
8065
× Hassan, Mohd Ali |
|||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Cellulase production from bacteria can be an advantage as the enzyme production rate is normally higher due to bacterial high growth rate. Screening of bacteria, optimisation of fermentation conditions and selection of substrates are important for the successful production of cellulase. This study is conducted to produce cellulase from our local isolate Bacillus pumilus EB3, using carboxymethyl cellulose (CMC) as substrate. Following that, cellulase produced from Bacillus pumilus EB3 was purified using ion exchange chromatography with anion exchanger (HiTrap QXL) for characterisation of the cellulase. Cellulase was successfully produced in 2L stirred tank reactor (STR) with the productivity of 0.53, 3.08 and 1.78 U/L.h and the maximum enzyme activity of 0.011, 0.079 and 0.038 U/mL for FPase, CMCase and β-glucosidase respectively. Purification of cellulase from Bacillus pumilus EB3 using ion exchange chromatography showed that 98.7% of total CMCase was recovered. Protein separation was however based on subtractive separation where the contaminants were bound to the column instead of CMCase. Characterisation of the enzyme found that CMCase from Bacillus pumilus EB3 has a molecular weight range from 30-65 kDa and was optimally active at pH 6.0 and temperature 60℃. The CMCase also retained its activity over a wide pH range (pH 5.0–9.0) and temperature range (30-70℃). | |||||||||||||
| 備考 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Seminar on Engineering and Technology (SET2006), September 4-5, 2006, Putrajaya, Malaysia | |||||||||||||
| 書誌情報 |
International Journal of Engineering and Technology 巻 3, 号 1, p. 47-53, 発行日 2006 |
|||||||||||||
| 出版社 | ||||||||||||||
| 出版者 | Federation of Engineering Institutions of Islamic Countries（FEIIC） | |||||||||||||
| URI | ||||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://psasir.upm.edu.my/39433/ | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | ©2006 FEIIC | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Cellulase | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Bacillus pumilus EB3 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Production | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Productivity | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Purification | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Characterisation | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 6755 | |||||||||||||
