{"created":"2023-05-15T11:59:18.324796+00:00","id":5663,"links":{},"metadata":{"_buckets":{"deposit":"a0db7ce6-d385-4e76-9a72-3df7706e75e0"},"_deposit":{"created_by":18,"id":"5663","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"5663"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00005663","sets":["6:7"]},"author_link":[],"item_20_date_granted_61":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-06-28"}]},"item_20_degree_grantor_59":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"九州工業大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17104","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_20_degree_name_58":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（情報工学）"}]},"item_20_description_30":{"attribute_name":"目次","attribute_value_mlt":[{"subitem_description":"1 Introduction||2 Evaluation of usefulness of housefly larvae based on amino acid analysis||3 Preparation of housefly larvae water extract (HLWE) with multifunctional activities||4 Summary","subitem_description_type":"TableOfContents"}]},"item_20_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Many bioactive compounds have been studied for the prevention or treatment of various diseases. Oxidative stress has been confirmed to be related to many chronic diseases, including hypertension and diabetes mellitus. Hypertension is a major chronic adult disease affecting 40% population worldwide, and angiotensin-converting enzyme (ACE, EC 3.4.15.1) plays an important role in elevation in blood pressure. Type 2 diabetes mellitus (DM) is the most common form of diabetes, which account for approximately 90 to 95% of all the diagnosed cases, and dipeptidyl peptidase-IV (DPP-IV, EC 3.4.14.5) is involved in this symptom. Therefore, the development of materials which have antioxidant, ACE-inhibitory (antihypertension), and DPP-IV-inhibitory (antidiabetic) activities become important to the prevention or treatment of these chronic diseases. Although many chemically synthesized antioxidants, ACE inhibitors, and DPP-IV inhibitors have been developed, it is still unclear whether these chemically synthesized compounds are sufficiently safe for long-term dosing or whether these compounds may have side effects. Therefore, bioactive compounds derived from natural sources are needed. Bioactive compounds are usually derived from plants, aquatic animals, and mammals. Although many types of insects have been used as foods and protein sources, there are few reports on biological activities of insects. Insects are an abundant resource, that, though not well explored, is expected to be a solution to food security in the future. Among them, the housefly has a high reproductive ability and a short lifecycle; the larvae prefer organic wastes, such as garbage, animal manure, and can convert these wastes into value-added biomass rich in protein, chitin, and fat. Housefly larvae have been used clinically in traditional Chinese medicine for a long time, but little information is available on the bioactive compounds from housefly larvae. Although housefly larvae are considered high-quality protein resource, there are few reports that compare the amino acid composition of housefly larvae with other ideal protein sources. In recent years, several attempts have been reported on the treatment of livestock manure using bioconversion by housefly larvae, and these studies show the potential of housefly larvae for applications with economic and environmental benefits.In this study, first, the utility of housefly larvae as a protein source was investigated. Second, in order to increase its applicability, the fraction containing protein was extracted and its multifunctional bioactivities were investigated. In chapter 1, first, the expectation on insects as a natural source was expressed. Second, the housefly larvae, the larval bioconversion system, the utilities of housefly larvae, and the background and objectives of the current study were introduced. In chapter 2, the utility of housefly larvae was evaluated by amino acid analysis. The housefly larvae contained sufficient amounts of all essential amino acids, and the amino acid composition was comparable to that of fishmeal, beef, chicken, pork, casein, and hen egg. These results showed the potential of housefly larvae as a good protein source. In chapter 3, we prepared housefly larvae water extract (HLWE) using decoction method and explored the biological activities of the extract for the potential application of the extract as a functional food. HLWE showed significant antioxidant activity (75.4% at 5.00 mg/mL), ACE-inhibitory activity (half-maximal inhibitory concentration [IC50]  = 0.430 mg/mL), and DPP-IV-inhibitory activity ([IC50] = 3.52 mg/mL). We found that the low-molecular-weight constituents (< 6 kDa) in HLWE contributed to antioxidant and ACE-inhibitory activities, whereas the high-molecular-weight constituents (> 6 kDa) contributed to DPP-IV inhibition. Our results suggested that housefly larvae may provide a useful source of multifunctional protein. The results of this study clearly suggest the potential of housefly larvae as a protein resource for feed, food, and functional foods with multifunctional activities with antioxidant, ACE-inhibitory, and DPP-IV-inhibitory activities. Moreover, this is the first time that multifunctional activities such as antioxidant, ACE-inhibitory, and DPP-IV-inhibitory activity were clarified in insects. Our findings provide important insights into the biological activities and applications of housefly larvae.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_20_description_5":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_description":"九州工業大学博士学位論文 学位記番号：情工博甲第334号 学位授与年月日：平成30年6月28日","subitem_description_type":"Other"}]},"item_20_description_60":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"平成30年度","subitem_description_type":"Other"}]},"item_20_dissertation_number_62":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第334号"}]},"item_20_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18997/00006874","subitem_identifier_reg_type":"JaLC"}]},"item_20_text_34":{"attribute_name":"アドバイザー","attribute_value_mlt":[{"subitem_text_value":"前田, 衣織"}]},"item_20_version_type_63":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"李,  虎軍","creatorNameLang":"ja"},{"creatorName":"Li,  Hujun","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-03"}],"displaytype":"detail","filename":"jou_k_334.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jou_k_334.pdf","objectType":"fulltext","url":"https://kyutech.repo.nii.ac.jp/record/5663/files/jou_k_334.pdf"},"version_id":"93eef532-6c91-49fd-bd78-2945e4c8c924"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antioxidant activity","subitem_subject_scheme":"Other"},{"subitem_subject":"ACE-inhibitory activity","subitem_subject_scheme":"Other"},{"subitem_subject":"DPP-IV-inhibitory activity","subitem_subject_scheme":"Other"},{"subitem_subject":"Housefly larvae","subitem_subject_scheme":"Other"},{"subitem_subject":"Multifunctional activities","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"The potential usefulness of the housefly larvae (Musca domestica) as materials for the development of multifunctional nutraceuticals","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The potential usefulness of the housefly larvae (Musca domestica) as materials for the development of multifunctional nutraceuticals","subitem_title_language":"en"},{"subitem_title":"多機能性食品の開発におけるイエバエ幼虫の素材としての潜在的有用性","subitem_title_language":"ja"}]},"item_type_id":"20","owner":"18","path":["7"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-08-03"},"publish_date":"2018-08-03","publish_status":"0","recid":"5663","relation_version_is_last":true,"title":["The potential usefulness of the housefly larvae (Musca domestica) as materials for the development of multifunctional nutraceuticals"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-16T02:09:55.013305+00:00"}