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アイテム
Three-Motorized-Stage Cyclic Stretching System for Cell Monitoring Based on Chamber Local Displacement Waveforms
http://hdl.handle.net/10228/00007275
http://hdl.handle.net/10228/00007275971c6eb8-d614-4c13-8aae-5d646b229f3c
| 名前 / ファイル | ライセンス | アクション |
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app9081560.pdf (2.4 MB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2019-07-03 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Three-Motorized-Stage Cyclic Stretching System for Cell Monitoring Based on Chamber Local Displacement Waveforms | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
| 著者 |
Huang, Wenjing
× Huang, Wenjing× Zhang, Sheng× Ahmad, Belal× 川原, 知洋
WEKO
24892
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Researchers have developed a cell stretching device to mimic the in vivo mechanical environment in vitro in order to investigate cell mechanotransduction. Cyclic stretch is involved in lengthening and relaxation phases. Cells may respond to mechanical stimulation rapidly within a few seconds, and sudden disruption of cell cytoskeletons may also occur at any point in any phase of cyclic stretch. However, until now, no research has been done to establish a method of collecting cell images at the two phases of cyclic stretch. Because image processing is time-consuming, it is difficult to adjust focus and collect high-resolution images simultaneously at the two phases during the process. In this study, a three-motorized-stage system was developed to meet the requirements. The results demonstrated that linear compensation is effective for cell imaging, and it is applicable to have a feed-forward control method without image processing. A method was then developed to determine the maximum displacement of the target in the horizontal and vertical directions, and the linear compensation waveforms were designed using the C program automatically and immediately before stretching. Further, the cyclic stretch was applied to cells using the three motorized stages, and clear phase-contrast cell imaging (30 fps) were obtained almost at any point in time. Detailed cell changes such as sudden disruption of cell–cell junctions, not only long-term cell response, were observed. Therefore, our study established a methodology to greatly improve the time resolution of imaging of cyclic stretch for the research of detailed cellular mechanotransduction. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Applied Sciences 巻 9, 号 8, p. 1560, 発行日 2019-04-15 |
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| 出版社 | ||||||||||||||
| 出版者 | MDPI | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.3390/app9081560 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 2076-3417 | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by/4.0/ | |||||||||||||
| 権利情報 | Copyright (c) 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | cell | |||||||||||||
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| 主題Scheme | Other | |||||||||||||
| 主題 | cyclic stretch | |||||||||||||
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| 主題Scheme | Other | |||||||||||||
| 主題 | mechanobiology | |||||||||||||
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| 主題Scheme | Other | |||||||||||||
| 主題 | time-lapse | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 7795 | |||||||||||||
