@inproceedings{oai:kyutech.repo.nii.ac.jp:00006084,
 author = {Sato, Shinobu and 佐藤, しのぶ and Ohtsuka,  Keiichi and Honda,  Satoshi and Sato,  Yusuke and Takenaka, Shigeori and 竹中, 繁織},
 book = {Journal of Physics: Conference Series},
 issue = {1},
 month = {Dec},
 note = {Methylation frequently occurs in cytosines of CpG sites to regulate gene expression. The identification of aberrant methylation of certain genes is important for cancer marker analysis. The aim of this study was to determine the methylation frequency in DNA samples of unknown length and/or concentration. Unmethylated cytosine is known to be converted to thymine following bisulfite treatment and subsequent PCR. For this reason, the AT content in DNA increases with an increasing number of methylation sites. In this study, the fluorescein-carrying bis-acridinyl peptide (FKA) molecule was used for the detection of methylation frequency. FKA contains fluorescein and two acridine moieties, which together allow for the determination of the AT content of double-stranded DNA fragments. Methylated and unmethylated human genomes were subjected to bisulfide treatment and subsequent PCR using primers specific for the CFTR, CDH4, DBC1, and NPY genes. The AT content in the resulting PCR products was estimated by FKA, and AT content estimations were found to be in good agreement with those determined by DNA sequencing. This newly developed method may be useful for determining methylation frequencies of many PCR products by measuring the fluorescence in samples excited at two different wavelengths., India-Japan Expert Group Meeting on Biomolecular Electronics & Organic Nanotechnology for Environment Preservation (IJEGMBE 2015), 23–26 December, 2015, Fukuoka, Japan},
 pages = {012015-1--012015-9},
 publisher = {IOP Publishing},
 title = {DNA methylation detection based on difference of base content},
 volume = {704},
 year = {2015},
 yomi = {サトウ, シノブ and タケナカ, シゲオリ}
}