{"created":"2023-05-15T11:59:37.605797+00:00","id":6140,"links":{},"metadata":{"_buckets":{"deposit":"4cdad833-2812-4c84-b3de-0fd9dd701d2c"},"_deposit":{"created_by":18,"id":"6140","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"6140"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00006140","sets":["6:7"]},"author_link":[],"item_20_date_granted_61":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-06-28"}]},"item_20_degree_grantor_59":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"九州工業大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17104","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_20_degree_name_58":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（工学）"}]},"item_20_description_30":{"attribute_name":"目次","attribute_value_mlt":[{"subitem_description":"1 Introduction and Literature Review||2 General Materials and Method||3 Ability of Pseudomonas Aeruginosa PA14 in Degrading The C-30 Quorum Sensing Inhibitor||4 Adaptation of Quorum Sensing System in Pseudomonas Aeruginosa PA14 Under The Treatment of AiiM Lactonase Quorum Quenching||5 Effect of AiiM Lactonase In Methane Production During Anaerobic Digestion Process||6 Effect Of C-30 and Lysozyme in Methane Production During Anaerobic Digestion Process||7 Concluding Remaks and Suggestion for Future","subitem_description_type":"TableOfContents"}]},"item_20_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Quorum sensing （QS） is a strategic system to control the production of virulent factors and enzymes. A general way to inhibit the bacterial QS is known as quorum quenching （QQ） which can be triggered by some inhibitors of QS （e.g. compounds and enzymes）. However, a recent study demonstrates that resistance toward the QQ occurs through a sequence of bacterial adaptation. Hence, the purpose of my Ph. D. study is to investigate the adaptation of bacteria under a treatment of QQ in the systems of monoculture and microbial consortia. In the monoculture system using Pseudomonas aeruginosa PA14, two main approaches were investigated to see the bacterial adaptation for 1） C-30 furanone as a QQ compound and 2） AiiM, a lactonase enzyme as a QQ enzyme. In the system of a microbial consortium, C-30 and AiiM were added to waste sewage sludge （WSS） to evaluate methane fermentation, which can be handled by the following hydrolysis, acidogenesis, and methanogenesis steps. In particular, the interaction between Gram-negative and Gram-positive bacteria could be important at the stages of hydrolysis and acidogenesis.  As a result, in the first approach of monoculture system, P. aeruginosa PA14 was able to degrade C-30 to C-56 according to the accumulation of bromine ions whereas a PA14 mutant showed less ability to degrade C-30. In addition, P. aeruginosa PA14 was able to utilize AiiM enzyme as a nutrient for the growth and some of the clinical isolates of P. aeruginosa showed a resistant ability towards AiiM by increasing the protease activity. In the microbial consortium using WSS, the addition of AiiM to WSS triggered a low methane production and lowered pH through the production of organic acids. In addition, the percentage of Gram-positive bacteria which may be functional as a repressor of methane fermentation increased in the presence of AiiM by changing the production of antimicrobial compounds in WSS with or without AiiM. The same result was obtained in the presence of C-30 at which a low percentage of Gram-negative bacteria was detected. Moreover, methane production increased in a condition that lysozyme was added to WSS.  Taken together, all the outcomes of this study should contribute to better understanding the adaptation of bacteria towards QQ along with the competitive relationship between  Gram-negative and Gram-positive  bacteria in the  bacterial consortia under the regulation of QS system.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_20_description_5":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_description":"九州工業大学博士学位論文 学位記番号:生工博甲第349号 学位授与年月日:令和元年6月28日","subitem_description_type":"Other"}]},"item_20_description_60":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"令和元年度","subitem_description_type":"Other"}]},"item_20_dissertation_number_62":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第349号"}]},"item_20_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18997/00007350","subitem_identifier_reg_type":"JaLC"}]},"item_20_text_34":{"attribute_name":"アドバイザー","attribute_value_mlt":[{"subitem_text_value":"前田, 憲成"}]},"item_20_version_type_63":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nguyen,  Phuong Thi Dong","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-28"}],"displaytype":"detail","filename":"sei_k_349.pdf","filesize":[{"value":"14.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"sei_k_349.pdf","objectType":"fulltext","url":"https://kyutech.repo.nii.ac.jp/record/6140/files/sei_k_349.pdf"},"version_id":"163b2790-ac99-4950-8db2-46f338819bee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Quorum Sensing","subitem_subject_scheme":"Other"},{"subitem_subject":"Quorum Quenching","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacterial Adaptation","subitem_subject_scheme":"Other"},{"subitem_subject":"Degradation","subitem_subject_scheme":"Other"},{"subitem_subject":"Microbial Consortia","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Bacterial Adaptation for the Inhibition of Quorum Sensing of Gram-Negative Bacteria","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bacterial Adaptation for the Inhibition of Quorum Sensing of Gram-Negative Bacteria","subitem_title_language":"en"},{"subitem_title":"グラム陰性細菌群菌のクォーラムセンシングの阻害に対する細菌学的適応機能","subitem_title_language":"ja"}]},"item_type_id":"20","owner":"18","path":["7"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-08-28"},"publish_date":"2019-08-28","publish_status":"0","recid":"6140","relation_version_is_last":true,"title":["Bacterial Adaptation for the Inhibition of Quorum Sensing of Gram-Negative Bacteria"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-16T02:11:34.497415+00:00"}