@article{oai:kyutech.repo.nii.ac.jp:00006234,
 author = {Yanagawa,  Katsunori and Shiraishi,  Fumito and Tanigawa,  Yusuke and Maeda, Toshinari and 前田, 憲成 and Mustapha,  Nurul Asyifah and Owari,  Satoko and Tomaru,  Hitoshi and Matsumoto,  Ryo and Kano,  Akihiro},
 issue = {11},
 journal = {Geosciences},
 month = {Oct},
 note = {Concretionary carbonates in deep-sea methane seep fields are formed as a result of microbial methane degradation, called anaerobic oxidation of methane (AOM). Recently, active microorganisms, including anaerobic methanotrophic archaea, were discovered from methane seep-associated carbonate outcroppings on the seafloor. However sedimentary buried carbonate nodules are a hitherto unknown microbial habitat. In this study, we investigated the microbial community structures in two carbonate nodules collected from a high methane flux site in a gas hydrate field off the Oki islands in the Sea of Japan. The nodules were formed around sulfate-methane interfaces (SMI) corresponding to 0.7 and 2.2 m below the seafloor. Based on a geochemical analysis, light carbon isotopic values ranging from −54.91‰ to −37.32‰ were found from the nodules collected at the shallow SMI depth, which were attributed to the high contributions of AOM-induced carbonate precipitation. Signatures of methanotrophic archaeal populations within the sedimentary buried nodule were detected based on microbial community composition analyses and quantitative real-time PCR targeted 16S rRNA, and functional genes for AOM. These results suggest that the buried carbonate nodule currently develops AOM-related microbial communities, and grows depending on the continued AOM under high methane flux conditions.},
 pages = {463-1--463-16},
 title = {Endolithic Microbial Habitats Hosted in Carbonate Nodules Currently Forming within Sediment at a High Methane Flux Site in the Sea of Japan},
 volume = {9},
 year = {2019},
 yomi = {マエダ, トシナリ}
}