{"created":"2023-05-15T11:59:49.090552+00:00","id":6422,"links":{},"metadata":{"_buckets":{"deposit":"ef0b17eb-3ce3-4f44-93d4-f06b72b466a6"},"_deposit":{"created_by":3,"id":"6422","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6422"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00006422","sets":["8:24"]},"author_link":["26918","958","26917"],"control_number":"6422","item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-09-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"219","bibliographicPageStart":"213","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Analytical Sciences"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In immunosensing, immobilization of the antibody on the sensing platform significantly influences the performance of the sensor. Herein, we propose a novel antibody-immobilization method based on a protein-polymer chain containing multiple copies of an antibody-binding protein, the Z-domain. In our approach, the Z-domain-containing polymer is prepared on the surface of the sensing platform with a biotinylation reaction from the archaeon Sulfolobus tokodaii. Biotinylation from S. tokodaii has a unique property by which biotin protein ligase (BPL) forms an extremely stable complex with its biotinylated substrate protein (BCCP). Here, we employed two types of engineered proteins: one was the fusion protein of BCCP with the Z-domain (BZB), in which BCCP was genetically attached to the N- and C-termini of the Z-domain; the other was a BPL dimer prepared by connecting two BPL molecules with a cross-linking reagent. We applied these two engineered proteins alternately onto the BPL-modified solid support of the surface plasmon resonance sensor chip, and succeeded in growing polymer chains comprising multiple units of BZB and the BPL dimer. The antibody-binding capability of the Z-domain-containing polymer thus prepared is adjustable by controlling the number of cycles of protein addition and the surface density of the polymer on the solid support.","subitem_description_type":"Abstract"}]},"item_21_description_60":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_21_link_62":{"attribute_name":"研究者情報","attribute_value_mlt":[{"subitem_link_text":"https://hyokadb02.jimu.kyutech.ac.jp/html/280_ja.html","subitem_link_url":"https://hyokadb02.jimu.kyutech.ac.jp/html/280_ja.html"}]},"item_21_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.2116/analsci.19P318","subitem_relation_type_select":"DOI"}}]},"item_21_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.2116/analsci.19P318"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2116/analsci.19P318","subitem_relation_type_select":"DOI"}}]},"item_21_relation_66":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"130007796150","subitem_relation_type_select":"NAID"}}]},"item_21_rights_13":{"attribute_name":"著作権関連情報","attribute_value_mlt":[{"subitem_rights":"2020 Copyright (c) The Japan Society for Analytical Chemistry"}]},"item_21_select_59":{"attribute_name":"査読の有無","attribute_value_mlt":[{"subitem_select_item":"yes"}]},"item_21_source_id_10":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10500785","subitem_source_identifier_type":"NCID"}]},"item_21_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0910-6340","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"1348-2246","subitem_source_identifier_type":"EISSN"}]},"item_21_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"578","subitem_subject_scheme":"NDC"}]},"item_21_text_28":{"attribute_name":"論文ID（連携）","attribute_value_mlt":[{"subitem_text_value":"10350240"}]},"item_21_text_36":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka 820-8502, Japan"},{"subitem_text_value":"Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka 820-8502, Japan"},{"subitem_text_value":"Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka 820-8502, Japan, Research Center for Bio-microsensing Technology, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata, Kitakyushu 8004-8550, Japan"}]},"item_21_text_63":{"attribute_name":"連携ID","attribute_value_mlt":[{"subitem_text_value":"8134"}]},"item_21_version_type_58":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyao, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uemura,  Utaro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26918","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Sueda, Shinji","creatorNameLang":"en"},{"creatorName":"末田, 慎二","creatorNameLang":"ja"},{"creatorName":"スエダ, シンジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Sueda","familyNameLang":"en"},{"familyName":"末田","familyNameLang":"ja"},{"familyName":"スエダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Shinji","givenNameLang":"en"},{"givenName":"慎二","givenNameLang":"ja"},{"givenName":"シンジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"958","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00325581","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000000325581"},{"nameIdentifier":"7005793664","nameIdentifierScheme":"Scopus著者ID","nameIdentifierURI":"https://www.scopus.com/authid/detail.uri?authorId=7005793664"},{"nameIdentifier":"280","nameIdentifierScheme":"九工大研究者情報","nameIdentifierURI":"https://hyokadb02.jimu.kyutech.ac.jp/html/280_ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"analsci.19P318.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"analsci.19P318.pdf","url":"https://kyutech.repo.nii.ac.jp/record/6422/files/analsci.19P318.pdf"},"version_id":"e01440bc-eb9d-423e-9e51-cf0ffb32b8af"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Immunosensor","subitem_subject_scheme":"Other"},{"subitem_subject":"antibody-binding protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Z-domain","subitem_subject_scheme":"Other"},{"subitem_subject":"surface plasmon resonance","subitem_subject_scheme":"Other"},{"subitem_subject":"biotinylation","subitem_subject_scheme":"Other"},{"subitem_subject":"protein interaction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal 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