{"created":"2023-05-15T12:00:16.926232+00:00","id":7027,"links":{},"metadata":{"_buckets":{"deposit":"5b4a800e-2dfd-4973-a104-dc8a6b23a165"},"_deposit":{"created_by":3,"id":"7027","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"7027"},"status":"published"},"_oai":{"id":"oai:kyutech.repo.nii.ac.jp:00007027","sets":["8:9"]},"author_link":["5963","30322","30323","30324","6749"],"control_number":"7027","item_1689815586683":{"attribute_name":"CRID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://cir.nii.ac.jp/crid/1390001204662138880","subitem_relation_type_select":"URI"}}]},"item_21_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-06-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"443","bibliographicPageStart":"437","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"分析化学","bibliographic_titleLang":"ja"},{"bibliographic_title":"BUNSEKI KAGAKU","bibliographic_titleLang":"en"}]}]},"item_21_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"テロメラーゼの触媒活性因子であるhuman telomerase reverse transcriptase（hTERT）遺伝子のプロモータ領域のメチル化パターンががんの進行と深くかかわっていることが報告されている．したがってこのプロモータ領域のメチル化パターンが検出できれば，がんの早期発見の手法として発展できると期待される．著者らは，hTERT遺伝子のプロモータ領域中の五つのCpGサイトを含む24塩基をターゲットとし，10種類のプローブDNAを有するマルチ電極チップを作製した．このチップを用いた電気化学的ハイブリダイゼーションアッセイ（Electrochemical hybridization assay, EHA）によってhTERT遺伝子のメチル化パターンによる口腔がん診断を試みた．ここでは，病院での簡易診断を目指して口腔がん，白板症，健常者から得られた口腔ぬぐい液を用いて，ゲノムを採取し，亜硫酸処理したのち，メチル化特異的ポリメラーゼ連鎖反応（Methylation-specific polymerase chain reaction, MSP）を行った．得られたMSP産物をEHAにより評価したところ，サンプルのメチル化頻度とがんの進行度に相関が示された．白板症，健常者を含むすべてのサンプルからメチル化頻度の高いDNAプローブに対する電気シグナルのReceiver Operating Characteristic（ROC）曲線を用いて口腔がんの正診断率を算出したところ，91％ であった．","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_21_link_62":{"attribute_name":"研究者情報","attribute_value_mlt":[{"subitem_link_url":"https://hyokadb02.jimu.kyutech.ac.jp/html/97_ja.html"}]},"item_21_publisher_7":{"attribute_name":"出版社","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会","subitem_publisher_language":"ja"}]},"item_21_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2116/bunsekikagaku.66.437","subitem_relation_type_select":"DOI"}}]},"item_21_rights_13":{"attribute_name":"著作権関連情報","attribute_value_mlt":[{"subitem_rights":"Copyright (c) The Japan Society for Analytical Chemistry 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