WEKO3
アイテム
Identification of Mycosporine-like amino acids and expression of 3-Dehydroquinate synthase gene in UV radiations-induced Deinococcus radiodurans R1
http://hdl.handle.net/10228/00008386
http://hdl.handle.net/10228/000083864144f897-7350-4320-9228-e21eec85a60b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
MJBMB2020_2_19-29.pdf (619.6 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2021-06-21 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Identification of Mycosporine-like amino acids and expression of 3-Dehydroquinate synthase gene in UV radiations-induced Deinococcus radiodurans R1 | |||||||||||||
| 言語 | en | |||||||||||||
| その他のタイトル | ||||||||||||||
| その他のタイトル | IDENTIFICATION OF MYCOSPORINE-LIKE AMINO ACIDS AND EXPRESSION OF 3-DEHYDROQUINATE SYNTHASE GENE IN UV RADIATIONS-INDUCED Deinococcus radiodurans R1 | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Huwaidi, Alaa
× Huwaidi, Alaa× Ahmad, Khairunisa Amira× Magdy, Mahmoud× Shaharuddin, Noor Azmi× 池野, 慎也
WEKO
8066
× Syahir, Amir |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Mycosporine-like amino acids (MAAs) are a group of more than 40 metabolites originated from 4-deoxygadusol featuring antioxidants, growth stimulation, and UV protective properties in many microorganisms. In D. radiodurans R1, 3-dehydroquinate synthase (DHQS) gene annotated in chromosome 1 encodes the precursor for all MAAs. In this study, a significant amount of MAAs were identified in D. radiodurans R1 after treatment with a different type of UV radiations, namely; the low energy UVA (360 nm) 6W and 100 W, and high energy UVC (254 nm) 6W at a period of 12 to 48 hours. The total RNA and MAAs were isolated from the UV-treated D. radiodurans R1. RT-qPCR experiment of the DHQS gene resulted in a significant increase of expression. Consequently, specific MAAs were identified using time-of-flight mass spectrometry (TOF-MS). They are mycosporine-taurine, mycosporine-glutamine, mycosporine-glutaminol, mycosporine-glutaminol-glucoside, mycosoprine-glycine, mycosporine-2-glycine, mycosporine-glycine:glutamic acid, shinorine, mycosporine-methylamine:serine, palythine-serine, and palythinol. The results suggested that these compounds play essential roles in D. radiodurans R1 radio-tolerance especially mycosporine-methylamine:serine and palythine-serine. This study can help to further understand the mechanism of radiation resistance in D. radiodurans R1, and its potential to be utilized as protective compound against radiation risk. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Malaysian Journal of Biochemistry & Molecular Biology 巻 23, 号 2, p. 19-29, 発行日 2020-08 |
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| 出版社 | ||||||||||||||
| 出版者 | Malaysian Society for Biochemistry and Molecular Biology | |||||||||||||
| URI | ||||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | https://msbmb2010.wixsite.com/mjbmb/august-2020 | |||||||||||||
| 日本十進分類法 | ||||||||||||||
| 主題Scheme | NDC | |||||||||||||
| 主題 | 467 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 2600-9005 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Deinococcus radiodurans | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | mycosporine-like amino acids | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | 3-dehydroquinate synthase | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | UV radiation | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | reactive oxygen species | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 研究者情報 | ||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/331_ja.html | |||||||||||||
| 論文ID（連携） | ||||||||||||||
| 値 | 10359378 | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 8751 | |||||||||||||
