WEKO3
アイテム
Functional expression of M3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae
http://hdl.handle.net/10228/1282
http://hdl.handle.net/10228/1282424fbb95-522a-4ed7-bc13-103eb3b044cb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
M3-30.pdf (429.8 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2009-02-13 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Functional expression of M3, a muscarinic acetylcholine receptor subtype, in taste bud cells of mouse fungiform papillae | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Eguchi, Kohgaku
× Eguchi, Kohgaku× 大坪, 義孝
WEKO
3998
× 吉井, 清哲 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Taste bud cells (TBCs) express various neurotransmitter receptors assumed to facilitate or modify taste information processing within taste buds. We investigated the functional expression of muscarinic acetylcholine receptor (mAChR) subtypes, M1-M5, in mouse fungiform TBCs. ACh applied to the basolateral membrane of TBCs elevates the intracellular Ca2+ level in a concentration-dependent manner with the EC50 of 0.6 μM. The Ca2+ responses occur in the absence of extracellular Ca2+, and are inhibited by atropine, a selective antagonist against mAChRs. The order of IC50s examined with a series of antagonists selective to mAChR subtypes show the expression of M3 on TBCs. Perforated whole-cell voltage-clamp studies show that 1 μM ACh blocks an outwardly rectifying current, and that 100 nM atropine reverses the block. RT-PCR studies suggest the expression of M3, but not the other mAChR subtypes. Immunohistochemical studies show that PLCβ2-immunoreactive TBCs and SNAP-25-immunoreactive nerve endings are immunoreactive to a transporter that packs ACh molecules into synaptic vesicles (VAChT). These results show that M3 occurs on a few fungiform TBCs, and suggest that a few nerve endings, and probably a few TBCs, release ACh by exocytosis. The role of ACh in taste responses is discussed. | |||||||||||||
| 書誌情報 |
Chemical Senses 巻 33, 号 1, p. 47-55, 発行日 2008-01-01 |
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| 出版社 | ||||||||||||||
| 出版者 | Oxford University Press | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1093/chemse/bjm065 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1464-3553 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0379-864X | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | This is a pre-copy-editing,author-produced PDF of an article accepted for publication in [Chemical Senses] following peer review.The definitive publisher-authenticated version [Chemical Senses 2008 33(1):47-55] is available online at: [http://chemse.oxfordjournals.org/cgi/content/abstract/33/1/47 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Ca2+ imaging | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | immunohistochemistry | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | pharmacology | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | RT-PCR | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | vesicular acetylcholine transporter | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | whole-cell clamp | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 業績ID | ||||||||||||||
| 値 | DA2FF9E8529C61924925755B0081BA64 | |||||||||||||
