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アイテム
Functional expression of ionotropic purinergic receptors on mouse taste bud cells
http://hdl.handle.net/10228/1283
http://hdl.handle.net/10228/128367ecc27f-21c1-4c05-b438-1d31a48cc964
| 名前 / ファイル | ライセンス | アクション |
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JOP_final.pdf (1.2 MB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2009-02-13 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Functional expression of ionotropic purinergic receptors on mouse taste bud cells | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Hayato, Ryotaro
× Hayato, Ryotaro× 大坪, 義孝
WEKO
3998
× 吉井, 清哲 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Neurotransmitter receptors on taste bud cells (TBCs) and taste nerve fibres are likely to contribute to taste transduction by mediating the interaction among TBCs and that between TBCs and taste nerve fibres. We investigated the functional expression of P2 receptor subtypes on TBCs of mouse fungiform papillae. Electrophysiological studies showed that 100 [mu m ATP applied to their basolateral membranes either depolarized or hyperpolarized a few cells per taste bud. Ca2+ imaging showed that similarly applied 1 mu m ATP, 30 [mu M BzATP (a P2X(7) agonist), or 1 mu M 2MeSATP (a P2Y(1) and P2Y(11) agonist) increased intraceflular Ca2+ concentration, but 100 mu M UTP (a P2Y(2) and P2Y(4) agonist) and alpha,beta-meATP (a P2X agonist except for P2X(2), P2X(4) and P2X(7)) did not. RT-PCR suggested the expression of P2X(2), P2X(4), P2X(7), P2Y(1), P2Y(13) and P2Y(14) among the seven P2X subtypes and seven P2Y subtypes examined. Immunohistostaining confirmed the expression of P2X2. The exposure of the basolateral membranes to 3 mm ATP for 30 min caused the uptake of Lucifer Yellow CH in a few TBCs per taste bud. This was antagonized by 100 mu M PPADS (a non-selective P2 blocler) and 1/mu M KN-62 (a P2X(7) blocker). These results showed for the first time the functional expression of P2X2 and P2X7 on TBCs. The roles of P2 receptor subtypes in the taste transduction, and the renewal of TBCs, are discussed. | |||||||||||||
| 書誌情報 |
Journal of Physiology 巻 584, 号 2, p. 473-488, 発行日 2007-10-01 |
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| 出版社 | ||||||||||||||
| 出版者 | Blackwell Publishing Ltd. | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1113/jphysiol.2007.138370 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0022-3751 | |||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1469-7793 | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | Copyright © 2007,Published on behalf of the Physiological Society | |||||||||||||
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| 権利情報 | The definitive version is available at www.blackwell-synergy.com. | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 業績ID | ||||||||||||||
| 値 | 0315DE4076D505444925755B008356F8 | |||||||||||||
| 情報源 | ||||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://www.blackwell-synergy.com/doi/abs/10.1113/jphysiol.2007.138370 | |||||||||||||
| 関連名称 | http://www.blackwell-synergy.com/doi/abs/10.1113/jphysiol.2007.138370 | |||||||||||||
