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Synthesis of a Peptide-Human Telomere DNA Conjugate as a Fluorometric Imaging Reagent for Biological Sodium Ion
http://hdl.handle.net/10228/00007295
http://hdl.handle.net/10228/000072958c5e0bc2-f439-4573-8c03-88f7db0d1851
名前 / ファイル | ライセンス | アクション |
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analsci.18SDP05.pdf (1.9 MB)
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Item type | 学術雑誌論文 = Journal Article(1) | |||||||||||||||||
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公開日 | 2019-07-18 | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | journal article | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | en | |||||||||||||||||
タイトル | Synthesis of a Peptide-Human Telomere DNA Conjugate as a Fluorometric Imaging Reagent for Biological Sodium Ion | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | eng | |||||||||||||||||
著者 |
佐藤, しのぶ
× 佐藤, しのぶ
WEKO
5963
× Imaichi, Yuuki× Yoshiura, Yukiko× Nakazawa, Koji× 竹中, 繁織
WEKO
6749
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抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | A peptide-oligonucleotide conjugate (1) was synthesized by the attachment of FAM, TAMRA, and biotin moieties to a telomere DNA sequence of 5′-TAG GGT TAG GGT TAG GGT TAG GG-3′. This conjugate was induced to be an anti-parallel structure in the presence of sodium ion (Na+), whereas a hybrid one was formed under potassium ion (K+) as a monitoring by circular dichromic spectra. The conformation change of this conjugate gave an effective FRET signal change upon the addition of NaCl, compared with the case of KCl. Under 5 mM KCl as an extracellular condition, a FRET change was observed upon addition of NaCl and quantitative FRET change was observed in 0 – 250 mM NaCl. This conjugate was immobilized on the cell surface through a sugar chain on the cell, biotinyl concanavallin A and streptavidin. This conjugate was utilized for Na+ sensing based on anti-parallel tetraplex formation with Na+. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
en : Analytical Sciences 巻 35, 号 1, p. 85-90, 発行日 2019-01-10 |
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出版社 | ||||||||||||||||||
言語 | ja | |||||||||||||||||
出版者 | 日本分析化学会 | |||||||||||||||||
DOI | ||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||
関連識別子 | https://doi.org/10.2116/analsci.18SDP05 | |||||||||||||||||
CRID | ||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||
識別子タイプ | URI | |||||||||||||||||
関連識別子 | https://cir.nii.ac.jp/crid/1390845713037967744 | |||||||||||||||||
日本十進分類法 | ||||||||||||||||||
主題Scheme | NDC | |||||||||||||||||
主題 | 434 | |||||||||||||||||
NCID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AA10500785 | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | EISSN | |||||||||||||||||
収録物識別子 | 1348-2246 | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 0910-6340 | |||||||||||||||||
著作権関連情報 | ||||||||||||||||||
権利情報 | The Japan Society for Analytical Chemistry | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Peptide-oligonucleotide conjugate | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | FAM | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | TAMRA | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | sodium ion | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | potassium ion | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | antiparallel tetraplex | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | hybrid tetraplex | |||||||||||||||||
キーワード | ||||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | fluorometric imaging | |||||||||||||||||
出版タイプ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
査読の有無 | ||||||||||||||||||
値 | yes | |||||||||||||||||
連携ID | ||||||||||||||||||
7809 |