WEKO3
アイテム
バイオセンシング用スクアライン色素系近赤外蛍光プローブの合理的設計と合成
https://doi.org/10.18997/0002001060
https://doi.org/10.18997/00020010600de7cce2-4354-4ad1-8bff-e00b0ca81bd2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
sei_k_501.pdf (7.3 MB)
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| アイテムタイプ | 学位論文 = Thesis or Dissertation(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-11-21 | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| タイトル | ||||||||
| タイトル | Rational Design and Synthesis of Squaraine Dye-Based Near-Infrared Fluorescent Probes for Biosensing | |||||||
| 言語 | en | |||||||
| タイトル | ||||||||
| タイトル | バイオセンシング用スクアライン色素系近赤外蛍光プローブの合理的設計と合成 | |||||||
| 言語 | ja | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| 著者 |
Gupta, Shekhar
× Gupta, Shekhar
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Biosensors, which convert biological responses into electrical signals, play a vital role in addressing this demand. They offer high specificity and independence from physical parameters such as pH and temperature, while also being environmentally friendly. The development of biosensors, including enzyme sensors, immune sensors, microbial sensors, and ion channel sensors, is a rapidly growing sector that is being actively promoted worldwide. The advantages of fluorescence-based detection technologies include their high sensitivity, selectivity, and ease of use. In addition, a range of fluorescent materials and sensing mechanisms can be employed in the systematic development of these techniques, thereby showcasing the adaptability of these approaches in detecting diverse targets with exceptional sensitivity and selectivity. Fluorescent biosensors are crucial for drug and cancer research. Photoinduced electron transfer (PET) and Förster Resonance Energy Transfer (FRET) processes play crucial roles in the detection of target biomolecules, such as bio-analytes and ions. These processes are utilized in various sensing platforms to achieve high sensitivity and selectivity in target detection. By considering the interplay between aggregation-caused quenching (ACQ), PET, and FRET processes, sensor design can be optimized to achieve robust and reliable detection of target biomolecules and ions. Integrating these mechanisms in sensor development allows for the creation of highly sensitive and selective platforms that are capable of overcoming challenges associated with fluorescence quenching and aggregation, ultimately advancing the field of bioanalytical and ion sensing. Using NIR fluorescent and quenchers in FRET biosensors enhances sensitivity for in vitro and in vivo imaging. Alignment of dye absorption and fluorescence emission wavelengths is key for optimal sensitivity. Adjustments to the enzyme active site peptide sequence ensure the quenching and fluorescent groups are within the Förster radius. Protein and peptide-based biosensors can be easily produced using synthetic chemistry techniques followed by enzymatic labeling with synthetic fluorophores. The assembly of near-infrared FRET biosensors requires the development of NIR dyes, namely fluorescent and quenching groups, as well as the design of an enzyme recognition site in proteases. The present thesis presents an insight into the design and synthesis of far-red sensitive squaraine dyes with different wavelengths. In this study, we developed novel squaraine-based far-red sensitive fluorescent peptide probes for chymotrypsin assays exploiting the intramolecular interaction between identical fluorophores as the homo-FRET mechanism. |
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| 目次 | ||||||||
| 内容記述タイプ | TableOfContents | |||||||
| 内容記述 | 1 Introduction| 2 Insturmentation and Characterization|3 Design and Synthesis of Novel Squaraine Dye with Highly Enhanced Far-Red Fluorescence and its Interaction with a Model Protein| 4 Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme| 5 Conclusion & Prospects | |||||||
| 備考 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 九州工業大学博士学位論文 学位記番号:生工博甲第501号 学位授与年月日:令和6年9月25日 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第501号 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（学術） | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2024-09-25 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 17104 | |||||||
| 学位授与機関名 | 九州工業大学 | |||||||
| 言語 | ja | |||||||
| 学位授与年度 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 令和6年度 | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| ID登録 | ||||||||
| ID登録 | 10.18997/0002001060 | |||||||
| ID登録タイプ | JaLC | |||||||
