WEKO3
アイテム
Novel Insights into Conformational Rearrangements of the Bacterial Flagellar Switch Complex
http://hdl.handle.net/10228/00007694
http://hdl.handle.net/10228/00007694801ac7ce-7bdb-4a25-916b-a9f78adb6b70
| 名前 / ファイル | ライセンス | アクション |
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10334472.pdf (2.4 MB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2020-04-03 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Novel Insights into Conformational Rearrangements of the Bacterial Flagellar Switch Complex | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Sakai, Tomofumi
× Sakai, Tomofumi× Miyata, Tomoko× Terahara, Naoya× Mori, Koichiro× Inoue, Yumi× 森本, 雄祐
WEKO
28693
× Kato, Takayuki× Namba, Keiichi× Minamino, Tohru |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The flagellar motor can spin in both counterclockwise (CCW) and clockwise (CW) directions. The flagellar motor consists of a rotor and multiple stator units, which act as a proton channel. The rotor is composed of the transmembrane MS ring made of FliF and the cytoplasmic C ring consisting of FliG, FliM, and FliN. The C ring is directly involved in rotation and directional switching. The Salmonella FliF-FliG deletion fusion motor missing 56 residues from the C terminus of FliF and 94 residues from the N terminus of FliG keeps a domain responsible for the interaction with the stator intact, but its motor function is reduced significantly. Here, we report the structure and function of the FliF-FliG deletion fusion motor. The FliF-FliG deletion fusion not only resulted in a strong CW switch bias but also affected rotor-stator interactions coupled with proton translocation through the proton channel of the stator unit. The energy coupling efficiency of the deletion fusion motor was the same as that of the wild-type motor. Extragenic suppressor mutations in FliG, FliM, or FliN not only relieved the strong CW switch bias but also increased the motor speed at low load. The FliF-FliG deletion fusion made intersubunit interactions between C ring proteins tighter compared to the wild-type motor, whereas the suppressor mutations affect such tighter intersubunit interactions. We propose that a change of intersubunit interactions between the C ring proteins may be required for high-speed motor rotation as well as direction switching. | |||||||||||||
| 書誌情報 |
mBio 巻 10, 号 2, p. e00079-19-1-e00079-19-14, 発行日 2019-04-02 |
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| 出版社 | ||||||||||||||
| 出版者 | American Society for Microbiology | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1128/mBio.00079-19 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 2150-7511 | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | Copyright (c) 2019 Sakai et al. | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | Creative Commons Attribution 4.0 International license. | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | https://creativecommons.org/licenses/by/4.0/ | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 研究者情報 | ||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/100001002_ja.html | |||||||||||||
| 論文ID（連携） | ||||||||||||||
| 値 | 10334472 | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 8193 | |||||||||||||
