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アイテム
Characteristics of A-type voltage-gated K+ currents expressed on sour-sensing type III taste receptor cells in mice
http://hdl.handle.net/10228/0002000502
http://hdl.handle.net/10228/0002000502e601baba-7145-4861-96a3-99d8e1cbd44d
| 名前 / ファイル | ライセンス | アクション |
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10429742.pdf (4.8 MB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||||
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| 公開日 | 2024-04-12 | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Characteristics of A-type voltage-gated K+ currents expressed on sour-sensing type III taste receptor cells in mice | |||||||||||||||
| 言語 | en | |||||||||||||||
| その他のタイトル | ||||||||||||||||
| その他のタイトル | Characteristics of A-type voltage-gated K#U+#UR currents expressed on sour-sensing type III taste receptor cells in mice | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 著者 |
Moribayashi, Takeru
× Moribayashi, Takeru
× Nakao, Yoshiki
× 大坪, 義孝
WEKO
3998
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | Sour taste is detected by type III taste receptor cells that generate membrane depolarization with action potentials in response to HCl applied to the apical membranes. The shape of action potentials in type III cells exhibits larger afterhyperpolarization due to activation of transient A-type voltage-gated K+ currents. Although action potentials play an important role in neurotransmitter release, the electrophysiological features of A-type K+ currents in taste buds remain unclear. Here, we examined the electrophysiological properties of A-type K+ currents in mouse fungiform taste bud cells using in-situ whole-cell patch clamping. Type III cells were identified with SNAP-25 immunoreactivity and/or electrophysiological features of voltage-gated currents. Type III cells expressed A-type K+ currents which were completely inhibited by 10 mM TEA, whereas IP3R3-immunoreactive type II cells did not. The half-maximal activation and steady-state inactivation of A-type K+ currents were 17.9 ± 4.5 (n = 17) and − 11.0 ± 5.7 (n = 17) mV, respectively, which are similar to the features of Kv3.3 and Kv3.4 channels (transient and high voltage-activated K+ channels). The recovery from inactivation was well fitted with a double exponential equation; the fast and slow time constants were 6.4 ± 0.6 ms and 0.76 ± 0.26 s (n = 6), respectively. RT-PCR experiments suggest that Kv3.3 and Kv3.4 mRNAs were detected at the taste bud level, but not at single-cell levels. As the phosphorylation of Kv3.3 and Kv3.4 channels generally leads to the modulation of cell excitability, neuromodulator-mediated A-type K+ channel phosphorylation likely affects the signal transduction of taste. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 書誌情報 |
en : Cell and Tissue Research 巻 396, p. 353-369, 発行日 2024-03-16 |
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| 出版社 | ||||||||||||||||
| 出版者 | Springer | |||||||||||||||
| DOI | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00441-024-03887-6 | |||||||||||||||
| NCID | ||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AA00600105 | |||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||
| 収録物識別子 | 0302-766X | |||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 1432-0878 | |||||||||||||||
| 著作権関連情報 | ||||||||||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||
| 権利情報 | Copyright (c) The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | In-situ whole-cell patch clamping | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Voltage-gated potassium currents | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Fungiform taste buds | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Sour-sensing cells | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Excitability | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
| 査読の有無 | ||||||||||||||||
| 値 | yes | |||||||||||||||
| 研究者情報 | ||||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/361_ja.html | |||||||||||||||
| 論文ID（連携） | ||||||||||||||||
| 値 | 10429742 | |||||||||||||||
| 連携ID | ||||||||||||||||
| 値 | 12089 | |||||||||||||||
