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アイテム
Adsorption of Laminin on Hydroxyapatite and Alumina and the MC3T3-E1 Cell Response
http://hdl.handle.net/10228/00006240
http://hdl.handle.net/10228/0000624079e2bfa1-5ed1-4047-a35c-1c8c0d1cd68c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ACSbse2_1162.pdf (514.7 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2017-06-22 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Adsorption of Laminin on Hydroxyapatite and Alumina and the MC3T3-E1 Cell Response | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Fujita, Hiroka
× Fujita, Hiroka× Kudo, Tada-aki× Kanetaka, Hiroyasu× 宮崎, 敏樹
WEKO
6369
× Hashimoto, Masami× Kawashita, Masakazu |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Artificial hydroxyapatite (HAp) is osteoconductive, but the mechanism is still unclear. It is likely that some serum proteins are adsorbed onto HAp and influence its osteoconductivity. We investigated the adsorption behavior of laminin (LN), which was isolated from murine Engelbreth–Holm–Swarm sarcoma, onto HAp and compared it with nonosteoconductive alpha-type alumina (α-Al2O3). Cell adhesion, spreading, and proliferation on native and LN-adsorbed discs of HAp or α-Al2O3 were examined using murine MC3T3-E1 osteoblastic cells. A larger amount of LN adsorbed onto HAp than α-Al2O3 despite the electrostatic repulsion between LN and HAp, suggesting the specific adsorption of LN onto HAp. The LN adsorbed onto HAp remarkably enhanced initial attachment and spreading of MC3T3-E1 cells, but subsequent proliferation of MC3T3-E1 cells was influenced by the type of material rather than LN adsorption. These fundamental findings imply that LN adsorbed on HAp could trigger osteoconductivity in vivo, aiding in the development of novel biomaterials that specifically adsorb LN and effectively enhance cell attachment and spreading. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : ACS biomaterials science & engineering 巻 2, 号 7, p. 1162-1168, 発行日 2016-06-22 |
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| 出版社 | ||||||||||||||
| 出版者 | American Chemical Society | |||||||||||||
| 言語 | en | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1021/acsbiomaterials.6b00190 | |||||||||||||
| NCID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA12739394 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 2373-9878 | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | American Chemical Society | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in ACS biomaterials science & engineering, copyright © American Chemical Society after peer review. | |||||||||||||
| 著作権関連情報 | ||||||||||||||
| 権利情報 | To access the final edited and published work, see http://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00190. | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | adsorption | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | bioactivity | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | hydroxyapatite | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | laminin; MC3T3-E1 | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | osteoconductivity | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | α-alumina | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 研究者情報 | ||||||||||||||
| URL | https://hyokadb02.jimu.kyutech.ac.jp/html/323_ja.html | |||||||||||||
| 論文ID（連携） | ||||||||||||||
| 値 | 10296953 | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 5470 | |||||||||||||
