WEKO3
アイテム
Artificial-enzyme gel membrane-based biosurveillance sensor with high reproducibility and long-term storage stability
http://hdl.handle.net/10228/00006411
http://hdl.handle.net/10228/0000641169439ce8-0e3f-4aa1-9756-5fba23da1e43
| 名前 / ファイル | ライセンス | アクション |
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an_134_2.pdf (611.6 kB)
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an_134_2_references.pdf (20.6 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2017-10-17 | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Artificial-enzyme gel membrane-based biosurveillance sensor with high reproducibility and long-term storage stability | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| 著者 |
池野, 慎也
× 池野, 慎也
WEKO
8066
× Yoshida, Tetsuya× 春山, 哲也
WEKO
20926
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| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | We propose that the most sophisticated strategy for primary biosurveillance is to exploit structural commonality through the detection of biologically relevant phosphoric substances. A novel assay, an artificial-enzyme membrane was designed and synthesized for sensor fabrication. This artificial-enzyme catalyzes the hydrolysis of the diphosphoric acid anhydride structure. This structure-selective, albeit not molecule-selective, catalytic hydrolysis was successfully coupled with amperometric detection. Since the catalytic reaction produces a dephosphorylation product (PO43−), it can be reduced by an electrode potential of −250 mV vs. Ag/AgCl. Owing to the structural selectivity of the artificial-enzyme membrane, the sensor can detect biological phosphoric substances comprehensively that have the diphosphoric acid anhydride structure. The sensor successfully determined various biological phosphoric substances at concentrations in the micromolar (µM) to millimolar (mM) range, and it showed good functional stability and reproducibility in terms of sensor responses. This sensor was used to detect Escherichia coli lysed by heat treatment, and the response increased with increasing bacterial numbers. This unique technique for analyzing molecular commonality can be applied to the surveillance of biocontaminants, e.g. microorganisms, spores and viruses. Artificial-enzyme-based detection is a novel strategy for practical biosurveillance in the front line. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 書誌情報 |
en : Analyst 巻 134, 号 2, p. 337-342, 発行日 2009-01-27 |
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| 出版社 | ||||||||||||||||||||||
| 出版者 | Royal Society of Chemistry | |||||||||||||||||||||
| DOI | ||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1039/B810846C | |||||||||||||||||||||
| ISSN | ||||||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||||||
| 収録物識別子 | 1364-5528 | |||||||||||||||||||||
| 著作権関連情報 | ||||||||||||||||||||||
| 権利情報 | Royal Society of Chemistry | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | artificial-enzyme membrane | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | bio-surveillance | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | dephosphorylation | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | electrochemical detection | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | PO43- | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | poly acrylamide | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||
| 査読の有無 | ||||||||||||||||||||||
| 値 | yes | |||||||||||||||||||||
| 連携ID | ||||||||||||||||||||||
| 値 | 625 | |||||||||||||||||||||
