WEKO3
アイテム
DNA methylation detection based on difference of base content
http://hdl.handle.net/10228/00007294
http://hdl.handle.net/10228/000072949a0cc18f-2727-47be-8de7-abc131e5eef9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1742-6596_704_1_012015.pdf (1.1 MB)
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| アイテムタイプ | 会議発表論文 = Conference Paper(1) | |||||||||||||||||
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| 公開日 | 2019-07-18 | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||||||||||||||
| 資源タイプ | conference paper | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | DNA methylation detection based on difference of base content | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| 著者 |
佐藤, しのぶ
× 佐藤, しのぶ
WEKO
5963
× Ohtsuka, Keiichi× Honda, Satoshi× Sato, Yusuke× 竹中, 繁織
WEKO
6749
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Methylation frequently occurs in cytosines of CpG sites to regulate gene expression. The identification of aberrant methylation of certain genes is important for cancer marker analysis. The aim of this study was to determine the methylation frequency in DNA samples of unknown length and/or concentration. Unmethylated cytosine is known to be converted to thymine following bisulfite treatment and subsequent PCR. For this reason, the AT content in DNA increases with an increasing number of methylation sites. In this study, the fluorescein-carrying bis-acridinyl peptide (FKA) molecule was used for the detection of methylation frequency. FKA contains fluorescein and two acridine moieties, which together allow for the determination of the AT content of double-stranded DNA fragments. Methylated and unmethylated human genomes were subjected to bisulfide treatment and subsequent PCR using primers specific for the CFTR, CDH4, DBC1, and NPY genes. The AT content in the resulting PCR products was estimated by FKA, and AT content estimations were found to be in good agreement with those determined by DNA sequencing. This newly developed method may be useful for determining methylation frequencies of many PCR products by measuring the fluorescence in samples excited at two different wavelengths. | |||||||||||||||||
| 備考 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | India-Japan Expert Group Meeting on Biomolecular Electronics & Organic Nanotechnology for Environment Preservation (IJEGMBE 2015), 23–26 December, 2015, Fukuoka, Japan | |||||||||||||||||
| 書誌情報 |
Journal of Physics: Conference Series 巻 704, 号 1, p. 012015-1-012015-9, 発行日 2015-12 |
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| 出版社 | ||||||||||||||||||
| 出版社 | IOP Publishing | |||||||||||||||||
| DOI | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | https://doi.org/10.1088/1742-6596/704/1/012015 | |||||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||||
| 収録物識別子 | 1742-6596 | |||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||
| 収録物識別子 | 1742-6588 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| 査読の有無 | ||||||||||||||||||
| 値 | yes | |||||||||||||||||
| 連携ID | ||||||||||||||||||
| 値 | 7808 | |||||||||||||||||
