WEKO3
アイテム
{"_buckets": {"deposit": "e4a4e98f-76b3-40b1-be3f-fce7635a601c"}, "_deposit": {"created_by": 3, "id": "5162", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "5162"}, "status": "published"}, "_oai": {"id": "oai:kyutech.repo.nii.ac.jp:00005162", "sets": ["9"]}, "author_link": ["7216", "20727", "20724", "20723", "20725", "20722", "20728", "20721"], "control_number": "5162", "item_21_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-05-16", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "7", "bibliographicPageEnd": "1145", "bibliographicPageStart": "1136", "bibliographicVolumeNumber": "7", "bibliographic_titles": [{"bibliographic_title": "Eukaryotic Cell"}]}]}, "item_21_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest. Here we report the isolation of a novel mutant, oda2-t, whose γ heavy chain is truncated at about 30% of the sequence. While the previously isolated γ chain mutant oda2 lacks the entire outer arm, oda2-t retains outer arms that contain α and β heavy chains, suggesting that the N-terminal sequence (corresponding to the tail region) is necessary and sufficient for stable outer-arm assembly. Thin-section electron microscopy and image analysis localize the γ heavy chain to a basal region of the outer-arm image in the axonemal cross section. The motility of oda2-t is lower than that of the wild type and oda11 (lacking the α heavy chain) but higher than that of oda2 and oda4-s7 (lacking the motor domain of the β heavy chain). Thus, the outer-arm dynein lacking the γ heavy-chain motor domain is partially functional. The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein.", "subitem_description_type": "Abstract"}]}, "item_21_description_60": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "Journal Article", "subitem_description_type": "Other"}]}, "item_21_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Microbiology"}]}, "item_21_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1128/EC.00102-08", "subitem_relation_type_select": "DOI"}}]}, "item_21_relation_14": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://ec.asm.org/cgi/content/abstract/7/7/1136", "subitem_relation_type_select": "URI"}}]}, "item_21_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "American Society for Microbiology"}]}, "item_21_select_59": {"attribute_name": "査読の有無", "attribute_value_mlt": [{"subitem_select_item": "yes"}]}, "item_21_source_id_8": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1535-9778", "subitem_source_identifier_type": "PISSN"}, {"subitem_source_identifier": "1535-9786", "subitem_source_identifier_type": "EISSN"}]}, "item_21_text_36": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "Department of Biological Sciences, Graduate School of Science, University of Tokyo"}, {"subitem_text_value": "Department of Bioscience and Bioinformatics, Kyushu Institute of Technology"}, {"subitem_text_value": "Department of Biological Sciences, Graduate School of Science, University of Tokyo"}, {"subitem_text_value": "Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center"}, {"subitem_text_value": "Department of Biological Sciences, Graduate School of Science, University of Tokyo/ Structural Biology, Graduate School of Science, Kyoto University"}, {"subitem_text_value": "Department of Bioscience and Bioinformatics, Kyushu Institute of Technology"}, {"subitem_text_value": "Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center"}, {"subitem_text_value": "Department of Biological Sciences, Graduate School of Science, University of Tokyo/ CREST, Japan Science and Technology Corporation"}]}, "item_21_text_63": {"attribute_name": "連携ID", "attribute_value_mlt": [{"subitem_text_value": "643"}]}, "item_21_version_type_58": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Liu, Zhongmei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20721", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takazaki, Hiroko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20722", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakazawa, Yuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20723", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakato, Miho", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20724", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yagi, Toshiki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20725", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Yasunaga, Takuo", "creatorNameLang": "en"}, {"creatorName": "安永, 卓生", "creatorNameLang": "ja"}, {"creatorName": "ヤスナガ, タクオ", "creatorNameLang": "ja-Kana"}], "familyNames": [{"familyName": "Yasunaga", "familyNameLang": "en"}, {"familyName": "安永", "familyNameLang": "ja"}, {"familyName": "ヤスナガ", "familyNameLang": "ja-Kana"}], "givenNames": [{"givenName": "Takuo", "givenNameLang": "en"}, {"givenName": "卓生", "givenNameLang": "ja"}, {"givenName": "タクオ", "givenNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "7216", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60251394", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000060251394"}, {"nameIdentifier": "7006317534", "nameIdentifierScheme": "Scopus著者ID", "nameIdentifierURI": "https://www.scopus.com/authid/detail.uri?authorId=7006317534"}, {"nameIdentifier": "0000-0002-2494-9603", "nameIdentifierScheme": "ORCiD", "nameIdentifierURI": "https://orcid.org/0000-0002-2494-9603"}, {"nameIdentifier": "266", "nameIdentifierScheme": "九工大研究者情報", "nameIdentifierURI": "https://hyokadb02.jimu.kyutech.ac.jp/html/266_ja.html"}, {"nameIdentifier": "7216", "nameIdentifierScheme": "Article by this author", "nameIdentifierURI": "https://kyutech.repo.nii.ac.jp/search?page=1\u0026size=20\u0026sort=wtl\u0026search_type=0\u0026q=\u0026wid=7216"}]}, {"creatorNames": [{"creatorName": "King, Stephen M.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20727", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamiya, Ritsu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "20728", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Eukaryotic Cell-2008-Liu-1136-45.pdf", "filesize": [{"value": "776.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 776100.0, "url": {"label": "Eukaryotic Cell-2008-Liu-1136-45.pdf", "url": "https://kyutech.repo.nii.ac.jp/record/5162/files/Eukaryotic Cell-2008-Liu-1136-45.pdf"}, "version_id": "7e5cd1f1-4054-48c6-8f2e-c94c529b29b3"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain", "subitem_title_language": "en"}]}, "item_type_id": "21", "owner": "3", "path": ["9"], "permalink_uri": "http://hdl.handle.net/10228/00006374", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-09-13"}, "publish_date": "2017-09-13", "publish_status": "0", "recid": "5162", "relation": {}, "relation_version_is_last": true, "title": ["Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain"], "weko_shared_id": -1}
Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain
http://hdl.handle.net/10228/00006374
http://hdl.handle.net/10228/00006374a62da522-5e2a-4b7d-b413-583853971252
名前 / ファイル | ライセンス | アクション |
---|---|---|
Eukaryotic Cell-2008-Liu-1136-45.pdf (776.1 kB)
|
|
Item type | 学術雑誌論文 = Journal Article(1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2017-09-13 | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
著者 |
Liu, Zhongmei
× Liu, Zhongmei× Takazaki, Hiroko× Nakazawa, Yuki× Sakato, Miho× Yagi, Toshiki× 安永, 卓生
WEKO
7216
× King, Stephen M.× Kamiya, Ritsu |
|||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest. Here we report the isolation of a novel mutant, oda2-t, whose γ heavy chain is truncated at about 30% of the sequence. While the previously isolated γ chain mutant oda2 lacks the entire outer arm, oda2-t retains outer arms that contain α and β heavy chains, suggesting that the N-terminal sequence (corresponding to the tail region) is necessary and sufficient for stable outer-arm assembly. Thin-section electron microscopy and image analysis localize the γ heavy chain to a basal region of the outer-arm image in the axonemal cross section. The motility of oda2-t is lower than that of the wild type and oda11 (lacking the α heavy chain) but higher than that of oda2 and oda4-s7 (lacking the motor domain of the β heavy chain). Thus, the outer-arm dynein lacking the γ heavy-chain motor domain is partially functional. The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein. | |||||||||||
書誌情報 |
Eukaryotic Cell 巻 7, 号 7, p. 1136-1145, 発行日 2008-05-16 |
|||||||||||
出版社 | ||||||||||||
出版者 | American Society for Microbiology | |||||||||||
DOI | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | info:doi/10.1128/EC.00102-08 | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 1535-9778 | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | EISSN | |||||||||||
収録物識別子 | 1535-9786 | |||||||||||
著作権関連情報 | ||||||||||||
権利情報 | American Society for Microbiology | |||||||||||
出版タイプ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
査読の有無 | ||||||||||||
値 | yes | |||||||||||
連携ID | ||||||||||||
643 | ||||||||||||
情報源 | ||||||||||||
識別子タイプ | URI | |||||||||||
関連識別子 | http://ec.asm.org/cgi/content/abstract/7/7/1136 |