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アイテム
Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain
http://hdl.handle.net/10228/00006374
http://hdl.handle.net/10228/00006374a62da522-5e2a-4b7d-b413-583853971252
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Eukaryotic Cell-2008-Liu-1136-45.pdf (776.1 kB)
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| アイテムタイプ | 学術雑誌論文 = Journal Article(1) | |||||||||||||
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| 公開日 | 2017-09-13 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 著者 |
Liu, Zhongmei
× Liu, Zhongmei× Takazaki, Hiroko× Nakazawa, Yuki× Sakato, Miho× Yagi, Toshiki× 安永, 卓生
WEKO
7216
× King, Stephen M.× Kamiya, Ritsu |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest. Here we report the isolation of a novel mutant, oda2-t, whose γ heavy chain is truncated at about 30% of the sequence. While the previously isolated γ chain mutant oda2 lacks the entire outer arm, oda2-t retains outer arms that contain α and β heavy chains, suggesting that the N-terminal sequence (corresponding to the tail region) is necessary and sufficient for stable outer-arm assembly. Thin-section electron microscopy and image analysis localize the γ heavy chain to a basal region of the outer-arm image in the axonemal cross section. The motility of oda2-t is lower than that of the wild type and oda11 (lacking the α heavy chain) but higher than that of oda2 and oda4-s7 (lacking the motor domain of the β heavy chain). Thus, the outer-arm dynein lacking the γ heavy-chain motor domain is partially functional. The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein. | |||||||||||||
| 書誌情報 |
Eukaryotic Cell 巻 7, 号 7, p. 1136-1145, 発行日 2008-05-16 |
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| 出版者 | American Society for Microbiology | |||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | info:doi/10.1128/EC.00102-08 | |||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 1535-9778 | |||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 1535-9786 | |||||||||||||
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| 権利情報 | American Society for Microbiology | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読の有無 | ||||||||||||||
| 値 | yes | |||||||||||||
| 連携ID | ||||||||||||||
| 値 | 643 | |||||||||||||
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| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://ec.asm.org/cgi/content/abstract/7/7/1136 | |||||||||||||
